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From IHC to multiplexing: A practical guide for modern tissue analysis

Learn how to transition from traditional IHC to multiplexed workflows and unlock deeper biological insights.

Immunohistochemistry (IHC) is a cornerstone of tissue analysis, but increasing research complexity is driving the need for more advanced, multiplexed approaches.

This eBook is designed for researchers already working with IHC who want to expand their analytical capabilities, deepen data insights, and streamline workflows.

What you’ll learn

  • The key limitations of traditional IHC workflows
  • The scientific advantages of multiplexing for tissue analysis
  • How to design effective multiplex panels
  • Best practices for antibody validation
  • Practical workflow considerations for implementation
  • Case studies demonstrating multiplexing in action

The solution

Discover how multiplexing enables the simultaneous analysis of multiple biomarkers within a single sample, providing richer spatial and biological context.

The eBook also introduces AtlasPlex as a practical, scalable solution to support multiplexed tissue analysis, from panel design through to data generation.

Download the eBook

Learn how to confidently transition from IHC to multiplexing - and enhance your research outcomes.

About Atlas Antibodies AB

Atlas Antibodies, founded on the Human Protein Atlas project, provides a comprehensive range of highly validated antibodies targeting over 75 % of the human proteome. Core products include Triple A Polyclonals advanced rabbit polyclonal antibodies, and PrecisA Monoclonals precise mouse monoclonals designed for superior specificity.

Atlas Antibodies also offer PrEST Antigens, recombinant protein fragments used for control experiments, and MolBoolean, an innovative technology for in situ protein-protein interaction studies. Manufactured in Sweden and distributed globally, Atlas Antibodies’ products support diverse research applications with exceptional accuracy and reliability.


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