mIRage-HSi is a sub-500 nm laser scanning O-PTIR microscope designed for imaging with ultra-high speed, with integrated fluorescence imaging and Raman spectroscopy.
- Thirty times quicker O-PTIR: Hyperspectral imaging completed in a matter of minutes, while single-wavelength imaging takes only seconds
- Sub-500 nm IR chemical spatial resolution
- Combined correlative imaging using fluorescence and Raman spectroscopy across multiple modalities
About mIRage-HSi
Next-level performance: Laser-scanning O-PTIR
The innovative mIRage-HSi represents the latest advancement in optical photothermal infrared (O-PTIR) spectroscopy and imaging, offering exceptional sub-500 nm infrared imaging alongside FTIR transmission and ATR-like spectroscopy.
This new technology introduces remarkable speed improvements, significantly shortening hyperspectral imaging from several hours to mere minutes and reducing the time required for single-frequency images from several minutes to just seconds.
This significant increase in imaging velocity broadens the applications of O-PTIR technology to include dynamic analyses, faster contaminant detection, and automated identification of submicron particles.
Regardless of the application (microplastics, pharma, life sciences, failure analysis, or materials science), the mIRage-HSi provides faster, more automated, and more efficient O-PTIR workflows.
Image Credit: Photothermal Spectroscopy Corp
Improved O-PTIR imaging measurement speed
mIRage-HSi: 16 seconds/image
30× speed improvement
- Tissue section: Imaged 1655 cm-1 (protein); field of view (FOV): 75x75 µm, 200 nm pixel size
Video Credit: Photothermal Spectroscopy Corp
Automated multi-wavelength imaging
Image sequence and RGB overlay
O-PTIR RGB overlay offers an automated technique for measuring user-selectable wavenumbers, resulting in an RGB overlay.
The RGB overlay illustration presents three specific wavenumbers that correspond to:
- 1080 cm-1 – Nucleic acid
- 1655 cm-1 – Proteins
- 1738 cm-1 – Lipids
Every wavenumber required 16 seconds.
Image Credit: Photothermal Spectroscopy Corp
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Preview mode: Real-time chemical imaging for ROI significant EoU and productivity improvement
The preview mode provides a fast chemical imaging display of a chosen area at a reduced resolution, offering a considerable improvement in measurement efficiency for identifying the desired feature.
Move, locate, image
Quickly identify the relevant ROI for defects, contaminants, and microplastics over an area greater than the objective FOV.
Video Credit: Photothermal Spectroscopy Corp
Application overview
13 seconds per image measurement time
High-speed chemical imaging of cheek cells
A: Brightfield image using a 50x, 0.8 NA objective. B: Single wavenumber IR chemical imaging RGB overlay, 1085 cm-1 DNA (blue), 1650 cm-1 protein (red), and 1743 cm-1 lipid (green). C: Zoomed-in image of B, showing detection of lipid droplets as small as 275 nm. D: O-PTIR Spectra (normalized to Amide I) from highlighted locations, spot #1 (lipid droplet) and spot #2 (nucleus). IR single-wavenumber images (as in B) are marked by an asterisk. Spectra are collected from a spot size of ∼500 nm.
Collection conditions: 50×50 µm measurement FOV, 100 nm pixel step size (250,000 pixels), 13 seconds per image, counter-prop mode. Spectral acquisition total time 8 sec 50x, 0.8 NA glass objective, tri-range IR QCL, 532 nm probe. Image Credit: Photothermal Spectroscopy Corp
17 seconds per image measurement time
High speed multi-spectral IR imaging of mouse brain tissue
A: Automated multiple (5) single-wavenumber IR chemical imaging RGB overlay: green (creatine) 1404/1655 cm-1, red (nucleic acid) 1080/1140 cm-1, and blue (lipid) 1738/1655 cm-1. B: Representative spectra from the highlighted locations, highlighting biochemistry from areas of chemical contrast as shown in A. IR image (A) wavenumber positions are marked by asterisks. Spectra are collected from a spot size of ∼500 nm.
Collection conditions: 150×150 µm measurement FOV, 250 nm pixel step size (360,000 pixels), 17 seconds per image, counter-prop mode with 50x, 0.8 NA glass objective, dual-range IR QCL, 532 nm probe. Sample courtesy of Dr. Mark Hackett, Curtin University, Australia. Image Credit: Photothermal Spectroscopy Corp