Porvair Sciences Microlute™ Protein Precipitation Plate

Testing site™

A trial was conducted by a drug metabolism department at a leading global pharmaceutical company in order to evaluate the various commercial protein precipitation plates currently available. The trial was conducted with a view to using the best performing product and recommending it in-house to other departments.

Porvair Sciences P3 Protein Precipitation Microplate

Image Credit: Porvair Sciences Ltd

Critical factors

During the evaluation, certain factors were critical to good separations:

Filter leakage

It is important that, when acetonitrile is added to denature the sample plasma protein, it does not immediately ‘wet out’ the filter and cause leakage from the filtration plate.

Flow rate

It is majorly advantageous to have a consistent flow rate of the sample when the vacuum is applied.

Non-specific binding of the compound to the plate/filter

By nature, some filters bind analytes and so choosing a non-binding filter material is essential as it maximizes the recovery of the analytes/compounds.

Method

  1. Place a MicroluteTM PPP onto a suitable collection plate (Product # 219250)
  2. Dispense 300ul acetonitrile into each well of the plate.
  3. Add 100ul of plasma sample to each well.
  4. Mix the solutions in each well by placing the plate on a plate shaker at 500 rev/min for 2 mins
  5. Centrifuge at 500 x g for 3 mins to collect supernatant in a clean collection plate

Note:

Recovery of compounds was completed, allowing for the effect of ion-suppression on the samples. A volume of 100 ml spiked plasma was used. This was compared against spiked standards.

In this experiment, the recovery accounts for both the recovery of the compound from the matrix and the suppression effect on the compound due to the matrix.

  • Vacuum manifold or positive pressure could be used instead of a centrifuge.
  • 3:1 ratio of acetonitrile to plasma sample is required.
  • Suitable for sera or plasma samples.

Results

Filter leakage

Microlute™ PPP showed no signs of leakage throughout the investigation. Three of the other manufacturers plates had significant leakage and so were ruled out at this stage.

Flow rate

Microlute™ PPP from Porvair Sciences demonstrated a consistent flow rate through the plate. Two of the manufacturers plates had significant blockages and so failed at this point.

Conclusions

After the evaluation, this department decided to use the Microlute™ PPP and recommended the plates to other departments for bioanalysis assays featuring protein precipitation. Factors that were important to this choice were:

  • Excellent Flow Rate
  • Clean System – No Leakage

About Porvair Sciences Porvair Sciences Limited

Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.

Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research. Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.


Sponsored Content Policy: News-Medical.net publishes articles and related content that may be derived from sources where we have existing commercial relationships, provided such content adds value to the core editorial ethos of News-Medical.Net which is to educate and inform site visitors interested in medical research, science, medical devices and treatments.

Last updated: May 19, 2020 at 9:17 AM

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Porvair Sciences Limited. (2020, May 19). Porvair Sciences Microlute™ Protein Precipitation Plate. News-Medical. Retrieved on October 04, 2024 from https://www.news-medical.net/whitepaper/20200519/Porvair-Sciences-Microlute-Protein-Precipitation-Plate.aspx.

  • MLA

    Porvair Sciences Limited. "Porvair Sciences Microlute™ Protein Precipitation Plate". News-Medical. 04 October 2024. <https://www.news-medical.net/whitepaper/20200519/Porvair-Sciences-Microlute-Protein-Precipitation-Plate.aspx>.

  • Chicago

    Porvair Sciences Limited. "Porvair Sciences Microlute™ Protein Precipitation Plate". News-Medical. https://www.news-medical.net/whitepaper/20200519/Porvair-Sciences-Microlute-Protein-Precipitation-Plate.aspx. (accessed October 04, 2024).

  • Harvard

    Porvair Sciences Limited. 2020. Porvair Sciences Microlute™ Protein Precipitation Plate. News-Medical, viewed 04 October 2024, https://www.news-medical.net/whitepaper/20200519/Porvair-Sciences-Microlute-Protein-Precipitation-Plate.aspx.

Other White Papers by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.