CD19, a B-cell surface protein, is expressed across B-cell development and it is expressed on almost all B-cell malignancies, such as ALL, chronic lymphocytic leukemia (CLL) and several non-Hodgkin lymphomas.
This universal expression and specificity for a single cell lineage have rendered CD19 an appealing target for antibody-based therapy, such as ADCs, bispecific antibodies, Fc-engineered antibodies and CAR-T cell therapy.
Until now, two anti-CD19 CAR-T cell therapies and one bispecific anti-CD19/CD3 antibody have received approval from the FDA for the treatment of hematologic B-cell malignancies. As per ClinicalTrials.gov, until May 7th, 2020, over 763 clinical trials have been launched.
To promote the development of CD19-targeted antibody-based therapy, ACROBiosystems has come up with an extensive range of CD19 protein products, such as biotinylated CD19, fluorescent-labeled CD19 and unconjugated CD19. Such proteins are ideal for antibody screening, immunization, antibody affinity measurement and detection of anti-CD19 CAR expression. The high activity of such proteins has been confirmed by ELISA, SPR and flow cytometry (FACS). Protocols are provided for free. This can help users save the development cycle.
ACROBiosystems has presented DMF for its recombinant CD19 to FDA and the DMF number has been filed as 034936. If DMF filed CD19 proteins from ACROBiosystems have been utilized in users’ drug development process, they can request that the company to offer DMF authorization to FDA in favor of submission or filing that users have made to the FDA.
Source: ACROBiosystems
| Molecule |
Cat. No. |
Host |
Product Description |
Structure |
| CD19 |
CD9-HP2H3 |
HEK293 |
PE-Labeled Human CD19 (20-291) Protein, His
Tag (Site-specific conjugation)Hot |
|
| CD9-HF2H2 |
HEK293 |
FITC-Labeled Human CD19 (20-291) Protein, His Tag DMF FiledHot |
|
| CD9-HF251 |
HEK293 |
FITC-Labeled Human CD19 (20-291) Protein, Fc Tag DMF FiledHot |
|
| CD9-HF2H3 |
HEK293 |
FITC-Labeled Human CD19 (20-291) Protein, His Tag Star Staining |
|
| CD9-H8259 |
HEK293 |
Biotinylated Human CD19, Fc Tag, ultra
sensitivity (primary amine labeling) DMF Filed |
|
| CD9-H82F7 |
HEK293 |
Biotinylated Human CD19 (20-291) Protein, Fc, Avitag™Hot |
|
| CD9-H82E9 |
HEK293 |
Biotinylated Human CD19 (20-291) Protein, His, Avitag™ (SPR verified)Hot |
|
| MBS-K005 |
|
Human CD19-coupled Magnetic Beads |
|
| CD9-H5251 |
HEK293 |
Human CD19 (20-291) Protein, Fc Tag, low endotoxin (Super affinity, MALS verified) DMF FiledHot |
|
| CD9-H52H2 |
HEK293 |
Human CD19 (20-291) Protein, His Tag DMF FiledHot |
|
| CD9-H5259 |
HEK293 |
Human CD19 (20-291) Protein, Fc Tag, low endotoxin |
|
| CD9-H5250 |
HEK293 |
Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin |
|
| CD9-H5258 |
HEK293 |
Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag |
|
| CD9-C5251 |
HEK293 |
Cynomolgus / Rhesus macaque CD19 (20-292) Protein, Fc Tag |
|
| CD9-C5221 |
HEK293 |
Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His Tag |
|
Product features
Detection of anti-CD19 CAR expression by FACS
1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3). PE Streptavidin was used as negative control (QC tested). Image Credit: ACROBiosystems
High affinity verified by SPR
Biotinylated Human CD19 (20-291), His, Avitag (Cat. No. CD9-H82E9) captured on Biotin CAP - Series S sensor Chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.255 nM as determined in an SPR assay (Biacore 8K) (QC tested). Image Credit: ACROBiosystems
Higher binding affinity than competitive products verified by ELISA, FACS, and SPR
Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the ELISA analysis against FMC63 Mab. The result showed that ACRO’s FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor. Image Credit: ACROBiosystems
Binding activity of FITC-Labeled Human CD19, His Tag from two different vendors were evaluated in the flow cytometry analysis against anti-CD19-CAR-293 cells. The result showed that ACRO's FITC-Labeled Human CD19, His Tag has a much higher binding activity than that of the other vendor. Image Credit: ACROBiosystems
Binding activity of Human CD19, His Tag from two different vendors were evaluated by SPR assay against FMC63 MAb. The result showed that ACRO's Human CD19, His Tag can bind FMC63 MAb with an affinity constant of 2.95 nM which is much higher than that of the other vendor. Image Credit: ACROBiosystems