The DynaPro® NanoStar® is a cuvette-based dynamic light scattering (DLS) instrument used for the analysis of the size and size distributions of peptides, proteins, polymers, nanoparticles and emulsions. A separate, optimized static light scattering (SLS) channel offers true molar mass measurements.

The NanoStar is among the most sensitive, robust and reliable DLS detectors available for protein and nanoparticle analysis.

Features

  • Sensitivity: 0.1 mg/mL of 14.4 kDa lysozyme
  • Hydrodynamic radius (Rh) range: 0.2 – 2500 nm
  • Molar mass range: 300 Da – 1 MDa
  • Temperature control: heated/cooled -15 to  +150°C,
  • Minimum sample requirement: 1.25 µL in quartz microcuvette, 4 µL in disposable microcuvette
  • Optional infrared laser wavelength to eliminate fluorescence
  • Can connect via optical fiber to a MALS detector for simultaneous online MALS and DLS measurement with size exclusion chromatography (SEC) or field-flow fractionation (FFF)
  • Touch-panel display for immediate access to data, diagnostics and settings
  • Software: DYNAMICS® analysis software offers comprehensive analysis of size and size distributions, molar mass, diffusion interaction parameter kD, second virial coefficient A2, thermal behavior Tagg/Tm/Tonset, DLS-based viscosity; full export of all results; optional 21CFR11 support for GMP

Applications

  • Size, aggregation and stability of small molecules, peptides, proteins, vesicles, nanoparticles, quantum dots and low/sub-micron colloids
  • Protein quality control for interaction or structural studies
  • Protein and polymer weight-average molecular weight
  • Macromolecular interactions: diffusion interaction parameter kD and second virial coefficient A2 (B22)
  • Thermal onset parameters Tagg, Tonset, Tm
  • Solubility of polymers
  • Critical micelle concentration and temperature
  • Nanoparticle structure by combining MALS and DLS sizing