Elsa: Cryo-transfer holder for cryo-EM and tomography

The Elsa cryo-transfer holder represents the next generation of cryogenic technology tailored for high-resolution cryo-electron microscopy (cryo-EM) and tomography experiments.


The Elsa cryo-transfer holder is as a cutting-edge, single-tilt liquid nitrogen holder purpose-built for the frost-free transfer of samples at liquid nitrogen temperature into a transmission electron microscope (TEM). Its primary application lies in the imaging of radiation-sensitive, frozen-hydrated specimens, specifically tailored for single particle cryo-electron microscopy (cryo-EM) studies.

  • <1.5 nm/minute drift rate: Ensures that image quality is high during data collection.
  • Centrosymmetric design: Reduces settling and drift during tomography by maintaining the center of gravity during a stage tilt.
  • Extended hold time: Allows >9 hours below -145 °C and >8 hours of stable, high-resolution imaging.
  • Larger dewar, redesigned from the ground up: Increases the liquid nitrogen volume by 2.5x.
  • Resolve <2.3 Å features: Enables high-resolution imaging even under cryo-conditions.

The Elsa Cryo-Transfer Holder comes with two distinct tip configurations. The ultra-low profile option employs the Gatan Quickload clipring-free mechanism for secure specimen placement and offers the most extensive tilt range among side entry cryo-transfer holders available today (±80° tilt).

In contrast, the standard tip, widely regarded as the easiest for novice users, utilizes a clipring mechanism for sample security.

Breakthrough K3 result

Breakthrough K3 result. A 2.7 Å structure of the 20S Proteasome with the K3 camera and Elsa cryo-holder on a TF20. Data courtesy of Alexander Myasnikov, Michael Braunfeld, Yifan Cheng, and David Agard.

Webinar: 3DED: A brief overview of data collection, and data analysis

3DED: A brief overview of data collection, and data analysis. The determination of precise atomic arrangements in a crystal material is the key to understanding the structure-property relationship, and it will further facilitate synthetic designs of new materials. In the past decades, the structure determination of submicron/nanometer crystals has been achieved via 3D electron diffraction (3DED). This technique is also known as continuous rotational electron diffraction (CRED) or MicroED (microcrystal electron diffraction. Video Credit: Gatan, Inc.

Elsa Cryo-Transfer Workstation

Elsa Cryo-Transfer Workstation. Tutorial on how to load a cryo-sample, recover it, as well as dry the Elsa cryo-transfer workstation. Video Credit: Gatan, Inc.