GenCRISPR synthetic sgRNAs for targeted gene editing

In knockout and HDR-mediated knock-in experiments, GenCRISPR synthetic sgRNAs are effective for targeted gene editing. Compared to conventional plasmid or virus-based delivery systems, using sgRNA with Cas9 protein in the ribonucleic protein (RNP) system has additional benefits.


  • Extremely effective even with challenging primary cell types
  • DNA-free/transgene-free
  • Detected shortly after transfection at high levels
  • Suitable for both ex and in vivo studies
  • Ready-to-use sgRNA, no IVT or annealing required
  • Promptly removed from the cell to reduce side effects

Available in multiple grades to support editing needs

EasyEdit sgRNA

The easy yet effective one-step solution

Modified synthetic sgRNAs that facilitate simple and efficient gene editing.

Delivering in 96-well plates is an option that supports high throughput screening.

  • Most economical solution - ideal for early research and discovery
  • High editing efficiency - No matter what cell type
  • Flexible at scale - 1 to 1000+ sgRNAs 2 to 100+ nmol

SafeEdit sgRNA

Safeguard the cells

High-purity sgRNAs that have been modified and purified using HPLC to minimize cytotoxicity and off-target effects.

  • Guaranteed purity of higher than 90%
  • Impact on cell viability is minimal
  • Less off-targeting as a result of truncated guides
  • Suitable for stem cells and primary cells

Preclinical grade sgRNA

Extra purification and quality control

For both ex vivo and in vivo preclinical studies.

  • After HPLC, further C18 desalting is performed to remove inorganic salts
  • Sterile packaging and filling
  • CoA and TSE/BSE Certification
  • Delivery in 1-3 weeks

cGMP grade sgRNA

cGMP sgRNA-dedicated for IND and clinical phase I material production is now available from GenScript:

  • State-of-the-art production facility
    Clear suite with a class A isolator in a class C background.
  • Comprehensive QA/QC
    Proprietary NGS method for sgRNA identity verification
  • Trusted by scientific partners globally
    Delivered 30+ cGMP-grade batches successfully

cGMP sgRNA Production Workflow:

GenCRISPR synthetic sgRNAs for targeted gene editing

Image Credit: GenScript

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.