This article and associated images are based on a poster originally authored by Yuka Ryomoto, Mari Tsutsumi, Masato Kanemaki, and Shuji Ozawa and presented at ELRIG Drug Discovery 2026 in affiliation with the National Institute of Genetics.
This poster is being hosted on this website in its raw form, without modifications. It has not undergone peer review but has been reviewed to meet AZoNetwork's editorial quality standards. The information contained is for informational purposes only and should not be considered validated by independent peer assessment.
What can we learn by depleting a protein of interest within minutes?
1. Why acute protein depletion?
Does permanent gene knockout tell the whole story?
Image Credit: Image courtesy of Yuka Ryomoto et al., in partnership with ELRIG (UK) Ltd.
2. Control protein depletion in time
AID2 enables rapid, inducible, and reversible protein depletion.
Image Credit: Image courtesy of Yuka Ryomoto et al., in partnership with ELRIG (UK) Ltd.
- Rapid: Protein depletion within minutes
- Inducible: Temporal control with 5-Ph-IAA
- Reversible: Protein levels recover after removal
3. Rapid depletion of RAD21 in human cells
RAD21-mAID-Clover was rapidly depleted after 5-Ph-IAA addition in HCT116 cells.
Image Credit: Yesbolatova et al., Nat. Commun. 11, 5701 (2020), Fig. 3; source data
4. What can acute protein depletion reveal about the target?
Examine the phenotypic consequences of acute protein depletion.
A complementary approach to functional studies of candidate drug targets. Image Credit: Image courtesy of Yuka Ryomoto et al., in partnership with ELRIG (UK) Ltd.
Potential application: Functional evaluation of candidate drug targets
5. AID2-mediated protein depletion in vivo
AID2 also functions in mice.
Reporter depletion was observed in multiple tissues. Image Credit: Yesbolatova et al., Nat. Commun. (2020), Fig. 6b
6. Where could AID2 fit into drug discovery?
Potential applications
- Functional target studies: Phenotypic consequences of acute protein depletion
- Disease-related proteins: Functional studies in cells and mice
- Disease models: Conditional cellular and mouse models
- Essential protein: Timed Depletion and functional analysis
About National Institute of Genetics
The National Institute of Genetics (NIG), located in Mishima, Shizuoka, is a premier Japanese research institute specializing in life science research with genetic approaches. Established in 1949, NIG plays a central role in advancing basic and applied research in life sciences, particularly in the fields of molecular biology, evolutionary genetics, and bioinformatics. It operates several national research programs and databases, including the DNA Data Bank of Japan (DDBJ), and is a core facility for the National Bioresource Project (NBRP). NIG also promotes international collaboration and provides advanced education through its affiliation with the Graduate University for Advanced Studies (SOKENDAI).
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Last Updated: Oct 28, 2026