Scientists unravel how LPS prevents or promotes development of allergic disease

NewsGuard 100/100 Score

Immunologists have long been baffled by LPS, the bacterial lipopolysaccharide that helps form the cell walls of Gram-negative bacteria.

Many experiments show that exposure to LPS during exposure to environmental allergens protects against developing asthma or allergic disease. Yet other numerous experiments show that the presence of LPS during exposure to environmental allergens does the opposite -; it promotes the development of asthma and other allergic disease.

Now Beatriz León, Ph.D., and fellow University of Alabama at Birmingham researchers have cut this Gordian knot in a study published in Cell Reports. They detail a series of mechanistic steps that reveal a surprising answer -; the key to LPS' promoting or preventing the allergic reaction lies in the allergen itself.

Greater understanding of the mechanisms underlying sensitization to allergic disease can offer new strategies to control allergic airway disease, especially asthma. A global epidemic of asthma, including a sharp increase in developed countries since the 1960s, affects about 300 million children and adults worldwide.

León and her UAB colleagues unraveled a complex trail for the antagonistic mechanisms of LPS to promote or prevent allergic disease through activation or suppression of T helper-2 immune cells.

Here are their findings, which also define specific roles for immune cells called classical monocytes and non-classical monocytes.

First, the presence of cysteine protease enzymatic activity in an airborne allergen is required for bacterial LPS to have its protective effect. Allergens that contain cysteine protease enzymatic activity -; like house dust mites or papain, an enzyme from papayas -; lead to prevention of sensitization in the presence of LPS. In contrast, the German cockroach airborne allergen, which lacks cysteine protease activity, promotes sensitization, despite the presence of LPS.

The cysteine protease acts through a still unknown mechanism to induce non-classical monocytes in the lungs to produce the cytokine GM-CSF, or granulocyte-macrophage colony-stimulating factor. GM-CSF signaling governs the ability of LPS to suppress T helper-2 responses to allergens like house dust mites.

Consequently, because the protective effects of LPS depend on GM-CSF, the beneficial effects of LPS are restricted to allergens that have cysteine protease activity."

Beatriz León, Ph.D., University of Alabama at Birmingham

In the absence of GM-CSF, LPS can favor pathogenic T helper-2 cell responses by supporting the trafficking of lung migratory dendritic cells into the lung-draining lymph nodes, where they can help initiate an immune response. However, when non-classical monocytes produce GM-CSF, LPS and GM-CSF synergize to differentiate classical monocytes into monocyte-derived dendritic cells that instruct the lung migratory dendritic cells for the suppression of T helper-2 cell allergic airway inflammation, through the production of interleukin-12.

"Our results show that GM-CSF segregates the opposed functions LPS has in the priming of allergen-specific T helper-2 cell responses," León said. "Different host sensitivities to GM-CSF and/or LPS -; influenced by genetic diversity or by environmental factors -; can therefore significantly affect the risk of allergic sensitization. Understanding these interactions may provide insight into future therapeutic interventions to circumvent and even reverse allergic disease."

The study, "GM-CSF production by non-classical monocytes controls antagonistic LPS-driven functions in allergic inflammation," involved different allergen sensitization in mouse models, transcriptional analysis, and in vivo loss-of-function and gain-of-function experiments.

Journal reference:

Kaur, K., et al. (2021) GM-CSF Production by Non-Classical Monocytes Controls Antagonistic LPS-driven Functions In Allergic Inflammation. Cell Reports.


The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Multiple atmospheric pollutants may be triggering asthma symptoms among children