Real-Time qPCR: Guidelines for a Comparison of Reagent Performance

Testing previously optimized qPCR assays with a new qPCR master mix requires careful experimental design to look at several factors of reagent performance including assay specificity, repeatability, linearity, sensitivity and efficiency.

Each of these qPCR assay performance indicators will be discussed with respect to how the qPCR master mix comparison experiments should be set up and how to analyze the data to assess each of these key factors of qPCR assays.


Bustin, S.A. et. al. (2009) The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. Clin Chem. 55, 1–12.


Sarah Teter, PhD, and Leta Steffen, PhD, Promega Corporation

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Last updated: Mar 29, 2019 at 7:29 AM

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