Testing previously optimized qPCR assays with a new qPCR master mix requires careful experimental design to look at several factors of reagent performance including assay specificity, repeatability, linearity, sensitivity and efficiency.
Each of these qPCR assay performance indicators will be discussed with respect to how the qPCR master mix comparison experiments should be set up and how to analyze the data to assess each of these key factors of qPCR assays.
Bustin, S.A. et. al. (2009) The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. Clin Chem. 55, 1–12.
Sarah Teter, PhD, and Leta Steffen, PhD, Promega Corporation
With a portfolio of more than 3,000 products covering the fields of genomics, protein analysis and expression, cellular analysis, drug discovery and genetic identity, Promega is a global leader in providing innovative solutions and technical support to life scientists in academic, industrial and government settings.
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