Real-Time qPCR: Guidelines for a Comparison of Reagent Performance

Introduction

Testing previously optimized qPCR assays with a new qPCR master mix requires careful experimental design to look at several factors of reagent performance including assay specificity, repeatability, linearity, sensitivity and efficiency.

Each of these qPCR assay performance indicators will be discussed with respect to how the qPCR master mix comparison experiments should be set up and how to analyze the data to assess each of these key factors of qPCR assays.

Reference:

Bustin, S.A. et. al. (2009) The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments. Clin Chem. 55, 1–12.

Acknowledgements

Sarah Teter, PhD, and Leta Steffen, PhD, Promega Corporation

About PromegaPromega Corporation

With a portfolio of more than 3,000 products covering the fields of genomics, protein analysis and expression, cellular analysis, drug discovery and genetic identity, Promega is a global leader in providing innovative solutions and technical support to life scientists in academic, industrial and government settings.

Promega products are used by life scientists who are asking fundamental questions about biological processes as well as by scientists who are applying scientific knowledge to diagnose and treat diseases, discover new therapeutics, and use genetics and DNA testing for human identification.


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Last updated: Feb 21, 2018 at 5:02 AM

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