This Quick Protocol provides instructions for use of the Maxwell® RSC PureFood Pathogen Kit with the Maxwell® RSC Instrument to purify pathogen DNA from food samples. For detailed instructions, including information on instrument setup and troubleshooting, please refer to the Maxwell® RSC PureFood Pathogen Kit Technical Manual #TM528, available at: www.promega.com/protocols/
Materials to be Supplied by the User
- microcentrifuge tubes, 1.5 ml or 2.0 ml
- sterile, aerosol-resistant pipette tips
- ThermoMixer® heat block
- Stomacher® lab paddle or vortexer
- Stomacher® bags
- 25 g of food sample
Download the full article to see the protocol for bacterial lysis from food matrix and automated DNA purification
Download the Full Article
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