Cryopreservation methods for diverse cell types

This article provides a comprehensive review of various cell lines successfully cryopreserved using Bambanker. With data from many published studies, it demonstrates the effectiveness and adaptability of Bambanker in preserving various cell lines.

Summary

Cryopreservation of mammalian cells is an important procedure in biological research because it protects valuable cell lines from contamination or equipment failure.

Several problems can develop when suitable freezing equipment is unavailable or when serum, additional wash procedures, or complex freezing methods introduce undesirable variables.

Bambanker is a ready-to-use cryoprotectant that simplifies freezing and reduces workflow complexity, allowing labs to standardize conditions and increase repeatability.

It has been documented in peer-reviewed publications for the cryopreservation of over 120 cell types and samples. This article collects published examples into a DOI-linked table for easy reference.

Materials and methods

This article analyzes peer-reviewed scientific papers that mentioned Bambanker as a cryopreservation medium. Publications were screened and reviewed to obtain the preserved cell type/sample type and the accompanying reference information shown in Table 1. The literature search and selection procedure is outlined as follows:

  • Databases searched: PubMed, Google Scholar
  • Time window: Up to December 2025
  • Inclusion criteria:
    • Peer-reviewed publications
    • Bambanker use is described in Methods/Materials
    • DOI
  • Selection approach
    • References were located through a literature review and included if Bambanker was specifically mentioned as the cryopreservation medium. To make the table brief and useful, one representative publication was chosen for each cell type/sample type.
  • Update policy
    • This table is reviewed and updated annually to include newly published cell types and samples. The present version covers literature available up until December 2025.

Cryopreservation methods for diverse cell types

Image Credit: NIPPON Genetics EUROPE GmbH

Results

Peer-reviewed literature revealed many instances where Bambanker was successfully employed to cryopreserve distinct cell lines.

To demonstrate these findings, Table 1 shows one article per cell line as an example. Each entry provides the cell type and its DOI, allowing researchers to quickly access papers that demonstrate Bambanker's efficacy across diverse cell types.

Table 1. Summary of cell lines successfully cryopreserved with Bambanker. Source: NIPPON Genetics EUROPE GmbH

Cell/sample type Species DOI
Immune and hematopoietic
Bone marrow mononuclear cells (MNC) Yorkshire miniswine https://doi.org/10.1111/j.1540-8191.2010.01086.x
CAR T cells Human https://doi.org/10.1038/s41586-025-09507-9
CD3+ T Human https://doi.org/10.1007/s00262-012-1375-5
CD34+ hematopoietic stem cells Human https://doi.org/10.1016/j.omtm.2017.11.008
CD4 T cells Human https://doi.org/10.1016/j.immuni.2024.11.004
CD8+ T Human https://doi.org/10.1038/s41586-024-07300-8
Hematopoietic progenitor
cells (HPCs)
Human https://doi.org/10.1186/s13024-018-0297-x
Jurkat Human https://doi.org/10.1038/s41586-025-09433-w
Leukocytes Human https://doi.org/10.3390/cells10040843
Lung interstitial cells (Mø) Mouse https://doi.org/10.3390/biomedicines9091241
Lymphoblast cell line (LCLs) Human https://doi.org/10.1371/journal.pone.0174317
Macrophage cell line RAW 264.7 Mouse https://doi.org/10.1093/jac/dks056
Naive CD4+ T cells Human https://doi.org/10.1093/cei/uxab012
Naive CD8+ T cells Human https://doi.org/10.1093/cei/uxab012
Peripheral blood mononuclear cells (PBMCs) Human https://doi.org/10.3892/ol.2023.13967
Spleen cells Human https://doi.org/10.1016/j.cell.2019.09.035
Splenocytes Mouse https://doi.org/10.1016/j.cell.2019.09.035
Tonsillar cells Human https://doi.org/10.1093/ndt/gfr403
Type-2 conventional dendritic cells (cDC2s) Human https://doi.org/10.1016/j.cell.2019.09.035
U-937 Human https://doi.org/10.1007/s00418-025-02368-3
Uterine tissue-resident macrophages (Mø) Mouse https://doi.org/10.3390/biomedicines11030985
Stem, progenitor, and differentiated derivatives
Adipose-derived stem cells (ASCs) Human https://doi.org/10.1038/nprot.2010.199
Airway basal stem cell (ABSC) Human https://doi.org/10.1186/s12931-025-03302-w
Bone marrow-derived endothelial progenitor cells (EPCs) Yorkshire miniswine https://doi.org/10.1016/j.athoracsur.2007.12.006
Bone marrow-derived mesenchymal stem cells (BM-MSCs) Horse https://doi.org/10.1186/scrt483
Embryonic stem cells (ESCs) Human https://doi.org/10.1016/j.stemcr.2023.01.007
Gingiva-derived mesenchymal stem cells (GMSCs) Human https://doi.org/10.3390/bioengineering5010008
Hepatoblast-like cells (HBCs) Human https://doi.org/10.1002/hep4.1111
hiPSC-derived cardiomyocytes Human https://doi.org/10.1152/physiolgenomics.00021.2020
hiPSC-derived microglia progenitors MG01 Human https://doi.org/10.3390/s41467-024-52400-8
iPSC-derived motor neurons Human https://doi.org/10.1016/j.nbd.2024.106673
Induced pluripotent stem cells (iPSCs) Human https://doi.org/10.1016/j.xpro.2023.102073
Induced pluripotent stem cells (iPSCs) Mouse https://doi.org/10.1247/csf.11008
iPSC-derived neural progenitor cells (NPCs) Human https://doi.org/10.21769/bioprotoc.3939
Mesenchymal stem cells (MSCs) Human https://doi.org/10.1371/journal.pone.0282473
Neural stem cells (NSCs) Human https://doi.org/10.1186/s12987-023-00471-y
Epithelial cells
Bronchial epithelial cells (BECs) Human https://doi.org/10.1371/journal.pone.0306197
Chinese hamster ovary cells (CHO-K1) Chinese hamster https://doi.org/10.3389/fphar.2019.00851
Large airway epithelial (LAE) cells Human https://doi.org/10.1016/j.celrep.2024.114076
MCF10A Human https://doi.org/10.1007/s00418-025-02368-3
MCF10AT1 Human https://doi.org/10.1007/s00418-025-02368-3
Medullary thymic epithelial cells Mouse https://doi.org/10.1084/jem.20240817
Nasal epithelial cells (HNECs) Human https://doi.org/10.1371/journal.pone.0306197
RPE1 (retinal pigment epithelial) Human https://doi.org/10.1038/s41586-025-09433-w
RWPE-1 Human https://doi.org/10.1007/s00418-025-02368-3
Small airway epithelial (SAE) cells Human https://doi.org/10.1016/j.celrep.2024.114076
Uterine epithelial cells (pbUEC) Bovine https://doi.org/10.1111/j.1439-0531.2011.01792.x
Fibroblasts & stromal
Dermal fibroblast BJ cells Human https://doi.org/10.1016/j.isci.2024.109708
Dermal fibroblast HFF-1 cells Human https://doi.org/10.1016/j.isci.2024.109708
Embryonic fibroblasts Canine https://doi.org/10.1016/j.theriogenology.2005.12.015
Embryonic fibroblasts Mouse https://doi.org/10.1016/j.theriogenology.2005.12.015
Fibroblasts Porcine https://doi.org/10.1111/j.1399-3089.2006.00365.x
Gastric cancer-associated fibroblasts (CAFs) Human https://doi.org/10.1016/j.xpro.2021.100553
Lung fibroblasts Human https://doi.org/10.1002/adfm.202515610
Oral fibroblasts Human https://doi.org/10.1016/j.bbrep.2021.101169
Periodontal ligament fibroblasts (PDLFs) Human https://doi.org/10.1002/cre2.533
Stromal cells of brain Mouse https://doi.org/10.1186/s12865-019-0314-z
Stromal cells of liver Mouse https://doi.org/10.1186/s12865-019-0314-z
Stromal cells of lung Mouse https://doi.org/10.1186/s12865-019-0314-z
Stromal cells of spleen Mouse https://doi.org/10.1186/s12865-019-0314-z
Stromal vascular fraction (SVF) Human https://doi.org/10.1002/jbm.a.37430
Cancer
Adenocarcinomic human alveolar basal epithelial cells A549 Human https://doi.org/10.1038/s41586-025-09433-w
Colon cancer cell line DLD1 Human https://doi.org/10.1016/j.ymeth.2019.04.010
Colon cancer cell line HCT116 Human https://doi.org/10.1016/j.ymeth.2019.04.010
Gastric tumor cells Human https://doi.org/10.1038/s41467-024-52615-9
HCC4006 Human https://doi.org/10.1038/s41586-025-09433-w
HeLa Human https://doi.org/10.1007/s00418-025-02368-3
HEK293T cells Human https://doi.org/10.1016/j.cmet.2021.11.001
KGN cell line Human https://doi.org/10.1158/0008-5472.can-24-2341
Liver cancer cell line HepG2 Human https://doi.org/10.18433/J3VK5G
LNCAP Human https://doi.org/10.1007/s00418-025-02368-3
MCF7 Human https://doi.org/10.1007/s00418-025-02368-3
MDA-MB-231 Human https://doi.org/10.1007/s00418-025-02368-3
MDA-MB-468 Human https://doi.org/10.1007/s00418-025-02368-3
NCI-H1048 Human https://doi.org/10.1038/s41586-025-09433-w
NCI-H1299 Human https://doi.org/10.1038/s41586-025-09433-w
NCI-H358 cells Human https://doi.org/10.1016/j.xcrm.2025.102317
NCI-H446 Human https://doi.org/10.1038/s41586-025-09433-w
NCI-H526 Human https://doi.org/10.1038/s41586-025-09433-w
NCI-H69 Human https://doi.org/10.1038/s41586-025-09433-w
NCI-H82 Human https://doi.org/10.1038/s41586-025-09433-w
Ovarian cancer cells SKOV-3 Human https://doi.org/10.3389/fphar.2019.00851
PC3 Chung Human https://doi.org/10.1007/s00418-025-02368-3
PC3 Guise Human https://doi.org/10.1007/s00418-025-02368-3
T47D Human https://doi.org/10.1007/s00418-025-02368-3
U-2 OS Human https://doi.org/10.1007/s00418-025-02368-3
Patient-derived samples
Ascites-derived HGSC patient-derived xenograft Human https://doi.org/10.1186/s13046-022-02570-4
Breast cancer tissue biopsies Human https://doi.org/10.1038/s42003-022-04025-0
Gastric cancer biopsies Human https://doi.org/10.1038/s41598-022-12610-w
Glioma tissue cells Human https://doi.org/10.1038/s41467-025-58452-8
Medulloblastoma tumor tissue Human https://doi.org/10.1038/s41467-025-56268-0
Ovarian cancer models (OCMs) Human https://doi.org/10.1186/s13073-021-00952-5
Ovarian metastatic cells Human https://doi.org/10.1038/s41467-024-52615-9
Ovarian single cells Human https://doi.org/10.1038/s41467-019-11036-9
Pancreatic ductal adenocarcinoma cells Human https://doi.org/10.1016/j.celrep.2019.01.048
Peritoneal metastases cells Human https://doi.org/10.1038/s41467-024-52615-9
Tumor breast biopsies Human https://doi.org/10.1016/j.xpro.2022.101712
Tumor colorectal biopsies Human https://doi.org/10.1016/j.xpro.2022.101712
Tumor gastric biopsies Human https://doi.org/10.1016/j.xpro.2022.101712
Tumor pancreatic biopsies Human https://doi.org/10.1016/j.xpro.2022.101712
Organoids and spheroids
Neurospheres glioblastoma stem-like cells (GSCs) Human https://doi.org/10.62347/grsp1268
Prostate organoid Human https://doi.org/10.1038/s41467-021-26901-9
Intestinal tumor-derived organoids Mouse https://doi.org/10.1007/978-1-0716-4023-4_6
NEPC patient-derived organoids Human https://doi.org/10.1002/1878-0261.12662
S1 fraction tumor-dependent spheroids Human https://doi.org/10.1016/j.xpro.2025.104286
Other
Adrenal cells Human https://doi.org/10.1210/clinem/dgac394
Atheroma-derived single-cell Human https://doi.org/10.1093/cvr/cvaf014
Bronchoalveolar lavage (BAL) fluid Human https://doi.org/10.1164/rccm.202310-1831le
C2C12 skeletal myoblasts Mouse https://doi.org/10.1371/journal.pone.0280527
Calvarial osteoblasts Mouse https://doi.org/10.1073/pnas.0709650104
Dental pulp-derived cells (pDPPCs) Porcine https://doi.org/10.1111/j.1432-0436.2008.00282.x
Fetal astrocytes Human https://doi.org/10.1007/s44192-023-00050-5
Fetal gonads Human https://doi.org/10.3390/cells10051214
Flip-In T-REx-293 cells Human https://doi.org/10.1016/j.cmet.2021.11.001
Hepatocytes Mouse https://doi.org/10.1016/j.cell.2018.11.012
Hippocampal tissue Mouse https://doi.org/10.1016/j.neuint.2020.104933
Hu5/KD3 myoblasts Human https://doi.org/10.1186/s13287-024-03922-x
Mitochondrial cardiomyopathy (MCM) heart tissues Human https://doi.org/10.1126/sciadv.adq1575
Mural granulosa cells (mGCs) Human https://doi.org/10.1016/j.rbmo.2025.104833
Myometrium cells Human https://doi.org/10.1371/journal.pone.0338485
Nuclei Human https://doi.org/10.41588-025-02188-0
Temporomandibular joint (TMJ) discs-derived cells Rat https://doi.org/10.1016/j.jobcr.2025.03.018
Testicular interstitial cells Mouse https://doi.org/10.3390/biomedicines10020487
Tumor brain cells Mouse https://doi.org/10.1016/j.xpro.2023.102049
Tumor spleen cells Mouse https://doi.org/10.1016/j.xpro.2023.102049

Conclusion

The examination of scientific literature demonstrates Bambanker's efficiency as a cryopreservation medium for a diverse spectrum of cell samples. Bambanker has been used consistently across multiple research studies and cell types, indicating its broad applicability.

Bambanker offers a streamlined cryopreservation technique that eliminates the need for serum, wash procedures, and complex protocols, making it the ideal solution for researchers looking to preserve valuable cell cultures.

Bambanker is a reliable cryopreservation medium for many cell types.

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Last updated: Aug 17, 2026 at 4:58 AM

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