The BioXplorer high-pressure range from H.E.L Group comprises fully automated bioreactor systems designed for gas-dependent bioprocesses, including syngas fermentation, microbial cultivation and cell-free biocatalysis.
Available as BioXplorer 100P, 400P and 5000P, the systems operate at pressures up to 10 bar, increasing the availability of dissolved gases and supporting the investigation and optimisation of gas-dependent bioprocesses.
The BioXplorer 100P and 400P offer parallel bioreactors for screening and process optimisation, while the BioXplorer 5000P provides larger working volumes for process development and scale-up.
Overview
Pressurized bioprocessing
Operating at elevated pressure increases gas solubility, improving gas availability for biological processes. This is particularly beneficial for gas fermentation, where the availability of gases such as hydrogen, carbon monoxide and carbon dioxide can influence microbial growth and productivity.
The BioXplorer high-pressure range provides automated pressure monitoring and control, allowing researchers to investigate the effects of pressure, gas composition and operating conditions on bioprocess performance.
Flexibility to optimize processes
The selection of probes for monitoring various physicochemical parameters in bioprocesses offers comprehensive insights. Taking advantage of the precise control and responsiveness of H.E.L's WinISO, process automation becomes achievable.
Feature and options
BioXplorer High-Pressure Range Comparison
 |
 |
 |
| BioXplorer 100P |
BioXplorer 400P |
BioXplorer 5000P |
Image Credit: H.E.L Group
| Specification Point |
BioXplorer 100P |
BioXplorer 400P |
BioXplorer 5000P |
| Number of independent parallel bioreactors |
8 |
4 |
1 |
| Bioreactor material |
Stainless steel body and lid |
Stainless steel body and lid |
Stainless steel cylinders |
| Total vessel volume |
200 mL |
500 ml |
Interchangeable 1-5 L vessels |
| Working volume |
50-150 mL |
120 – 400 ml |
1-5 L |
| Pressure rating |
Up to 10 bar |
Up to 10 bar |
Up to 10 bar |
| Sterilization |
Autoclavable bioreactors (including sensors) |
Autoclavable bioreactors (including sensors) |
- |
| Temperature Range |
|
0 – 135 °C |
−50 to 250 °C (circulator-dependent) |
| Temperature resolution / stability |
0.1 °C resolution |
0.1 °C resolution |
Typically within ±0.1 °C stability |
| Stirrer drive |
Magnetically driven captive impeller |
Magnetically driven captive impeller or Overhead agitation (magnetically coupled) |
Up to 2,000 rpm / 400 Ncm |
| Stirrer speed |
250–1,500 rpm |
Magnetic or overhead (magnetically coupled) |
Up to 2,000 rpm / 400 Ncm |
| Auto re-couple |
- |
- Magnetically Driven – Yes
- Overhead Agitation – N/A
|
N/A |
| Liquid additions with independent control for each bioreactor |
- |
2 standard; up to 2 additional (magnetic). With overhead: 2 standard; 1 additional |
Automated liquid feeds; volumetric syringe pumps |
| Gas additions |
2 sets of 8 MFCs standard; up to 2 additional sets |
2 sets of 4 MFCs standard; up to 2 additional sets |
Automated gas feeds; number of MFCs - |
| Standard options for MFC calibration |
Air, O2, N2, H2, CO, CO2 or CH4 |
- |
| Gas spargers |
2 |
- |
| Exhaust gas |
Peltier cooled condenser |
- |
| pH control |
1-way control (2-way control optional) |
Available |
| pH sensing |
0-14 |
Available |
| Polarographic DO sensing |
Optional |
Optional |
DO integration available |
| DO control |
- |
Gas/liquid flow rate, stirrer speed (Optional) |
COnfiguration-dependent |
| BioVIS – Understand cell density trends without taking samples |
Optional |
Optional |
Available |
| Tandem off-gas analysis |
Optional |
Optional |
Available (OUR, CPR, RQ) |
| Foam/Level detection |
Optional |
Optional |
Available; configuration-dependent |
| Sampling |
- |
Optional manual sampling |
Active pumps, dip tubes, automated level control or ASU |
| Control PC and software |
| WinISO control software (preinstalled) |
|
| WinISO control software (preinstalled) |
|
WinISO: step recipes, data logging, ELN export |
- Specification dependent on configuration or not confirmed. Contact H.E.L Group for further details. Availability of options depends on system configuration. Temperature performance is circulator-dependent.
The following features and options are available across the high-pressure BioXplorer range, unless otherwise specified.
Process Control and Flexibility
- Independent monitoring and control of temperature, pressure, pH, stirring and gas and liquid feeding.
- Automated liquid additions for pH regulation, nutrient feeding and process optimisation.
- Configurable gas supply strategies for aerobic and anaerobic bioprocesses.
- Optional sampling and process monitoring under pressure.
Intelligent Software Control and Analysis
- H.E.L's WinISO software provides automated experiment control, real-time monitoring and data logging.
- User-configurable experimental procedures with independent reactor control.
- Automated feedback control based on sensor measurements, time-based profiles or event detection.
- Data export for subsequent analysis.