The BioXplorer 100 from H.E.L is a multi-bioreactor system that has been specifically developed for the optimization of aerobic fermentations. Having a working volume as low as 50 mL, the BioXplorer 100 enables for low-working volume processes.
The bioreactor’s lid design allows customizable configurations, selecting from an extensive range of probes and feeds. Regulated by WinISO, BioXplorer has been developed to be a completely automatized system.
Overview
Customizable configurations
The BioXplorer 100 features eight configurable bioreactors, enabling working volumes varying from 50 to 150 mL. The reactors could be regulated separately or in parallel with a range of probe or sensor configurations available.
The modular design of the system enables accurate additions of liquids and gases, regulated by up to four sets of eight peristaltic pumps and also up to two sets of mass flow controllers.
Optimized solutions for a range of applications
The BioXplorer 100 has been improved for aerobic fermentation and optimal growth. The highly configurable system enables more in-depth process knowledge and extensive data, thereby allowing users to comprehend users processes in an improved way.
A huge benefit of the BioXplorer 100 is the high productivity from the eight parallel reactors, as they concurrently enable the screening and high throughput of several processes. Integrated with WinISO, data acquisition in real-time could be utilized for tracking the fermentation, and processes could be regulated live via the design of feedback loops.
Flexibility and control
BioXplorer 100 has been fitted with a highly customizable range of sensors improved for the process, thereby enabling for the acquisition of high-throughput data in real-time. BioXplorer 100 is regulated by H.E.L’s WinISO, enabling stringent control of the parameters of the bioprocess.
The automatization of processes depending on on-line measurements enables for response to changes in the bioreactor via feedback loops.
Compact parallel bioreactors
The BioXplorer 100’s compact bioreactors are both space and cost-effective. They enable running eight parallel bioprocesses within a small footprint. Furthermore, the system's low working volume (50 – 150 ml) reduces reagent expenses and shortens fermentation durations.
Even though small in size, the bioreactors have been developed to be user-friendly and for adaptability in sensor configuration to enable real-time data collection in real bioreactor settings. The BioXplorer 100 provides a smart solution to defeat bioprocessing challenges.
Features and options
Source: H.E.L Group
| Specification Point |
BioXplorer 100 |
| Number of independent parallel bioreactors |
8 |
| Bioreactor material |
Glass body, stainless steel lid |
| Total volume |
50-150 ml |
| Sterilization |
Autoclavable bioreactors (including sensors) |
| Temperature Range |
0 – 135 °C |
| Temperature resolution |
0.1 °C |
| Stirrer drive |
Magnetically driven captive impeller |
| Stirrer speed |
250 – 1500 rpm |
| Auto re-couple |
Yes |
| Liquid additions with independent control for each bioreactor |
2 as standard, options for upto 2 additional liquid feeds |
| Gas additions |
1 set of 8 mass flow controllers allowing independent control for each reactor as standard, option for 1 additional set |
| Standard options for MFC calibration |
Air, oxygen as option |
| Gas spargers |
1 |
| Exhaust gas |
Peltier cooled condenser |
| pH control |
1-way control (2-way control optional) |
| Polarographic DO sensing |
Standard |
| DO control |
Gas/liquid flow rate, stirrer speed |
| BioVIS – Understand cell density trends without taking samples |
Optional |
| Foam/Level detection |
Optional |
| Control PC and software |
Windows® PC with WinISO control software preinstalled:
- Fully independent control of all reactors
- Advanced control strategies for stirring and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection
- Real-time display and logging of all reaction parameters
- Easy export of data in a range of formats, including .csv
|
* Upper temperature limit for data acquisition from standard pH and DO probes is 60 °C and 40 °C, respectively. The lower temperature limit requires an integrated circulator option.