UV-Vis Spectrophometry explained

A method for measuring light absorption throughout the ultraviolet and visible regions of the electromagnetic spectrum is known as ultraviolet-visible (UV-Vis) spectrophotometry. Incident light can either be absorbed, reflected, or transmitted when it interacts with materials.

Atomic excitation, which describes the transformation of molecules from a low-energy ground state to an excited state, is brought on by the absorption of light in the UV-Vis range.

UV-Vis Spectrophometry explained

Image Credit: DeNovix Inc.

An atom must absorb enough radiation for electrons to migrate into higher molecular orbits before it can change excitation states. The absorption of shorter light wavelengths is often correlated with shorter bandgaps.

Therefore, the energy needed for molecules to go through these transitions is electrochemically unique. This concept can be used to quantify the analytes in a sample based on their absorption properties using a UV-Vis spectrophotometer.


Image Credit: DeNovix Inc.

Outlining UV-Vis spectrophotometers

A UV-Vis spectrophotometer gauges the amount of light passing through a sample in contrast to an incident light source reference measurement.

A pulsed Xenon flash lamp is used in the widely known DS-11 series of UV-Vis spectrophotometers from DeNovix to examine microvolume samples with light ranging in wavelength from 190 to 840 nm. A CCD optical detector with a wavelength precision of 0.5 nm collects the transmitted light.

The Beer-Lambert Law states that there is a linear relationship between the amount of light absorbed and the sample’s concentration.  The greater the distance the light travels through the sample (the pathlength), the more light is absorbed. 

By adjusting the analytical wavelengths and pathlength, UV-Vis spectrophotometers can consequently be used to calculate the concentration of certain analytes in a microvolume.

Unlike any other UV-Vis spectrophotometer currently on the market, the DS-11 is integrated with patented SmartPath®Technology, which enables accurate readings of samples over a greater dynamic range and ensures the optimum pathlength for each sample.

The ideal pathlength for each sample is determined using real-time absorbance, and automated modifications are then made to fit the detection range of the apparatus.

The DS-11 series also enables quartz or plastic cuvettes as well as microvolume measurements. The concentration, entire spectral output, and sample purity of proteins, single- and double-stranded DNA (ssDNA and dsDNA), and ribonucleic acid (RNA) can be evaluated quickly and automatically using either measurement mode.

DS 11 Series | Spectrophotometer | Fluorometer

UV-Vis spectrophotometers from DeNovix

DeNovix offers a number of UV-Vis spectrophotometer devices in the DS-11 series for the highest level of accuracy and dynamic range. Microvolume, cuvette, and fluorescence measuring modalities are among the models.

About DeNovix, Inc.

 

DeNovix Inc. is a Delaware based company that designs, manufactures and sells instrumentation for the life science industry. Founded in 2012, our focus is on providing innovative products and top-rated customer support. 

Our multi-award-winning products include the DS-11 Series Spectrophotometer / Fluorometer which was twice awarded Reviewers’ Choice Life Science Product of the Year as well as the prestigious Platinum Seal of Quality.  The DS-11 Series provides rapid, user-friendly UV-Vis and fluorometric quantification of DNA, RNA and proteins - cornerstone techniques in life science labs.

DeNovix’ latest innovation is the CellDrop™ Automated Cell Counter. CellDrop is the first instrument of its kind to enable cell counting without slides. Traditional cell counting requires disposable plastic slides or cumbersome manual counts using a hemocytometer and microscope. DeNovix’ patent-pending DirectPipette™ Technology features permanent sample surfaces that position the sample for counting and may be simply wiped clean to make ready for the next cell count. Laboratory waste and costs are reduced when labs use CellDrop.


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Last updated: Sep 1, 2022 at 5:27 AM

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