What is Fluorescence Force Microscopy?

Fluorescence force microscopy is a combination of atomic force microscopy and fluorescence microscopy, which allows molecular dynamics to be studied. The technique can be applied to single molecules, allowing a more in-depth study of protein folding, for example in cellular dynamics and cell movement.

Fluorescence Microscopy

Image Credit: Vshivkova/Shutterstock.com

Benefits of fluorescence force microscopy

As an overview, atomic force microscopy and fluorescence microscopy bring different strengths to the combined effect. Atomic force microscopy can reveal changes in the structure of individual cells, which can be correlated to cellular events seen by fluorescence microscopy. Combining their abilities allows molecules with fluorescent labels to be detected even in complex assemblies.

While a relatively new method, fluorescence force microscopy has been applied to several macromolecules, and other smaller molecules. The possibility to measure in real-time means that dynamic processes can be measured. For example, fluorescence force microscopy has been used to study the unfolding of one single ubiquitin protein in real-time.

In addition to cell dynamics, topographical cell changes can be seen in real-time. Events that typically occur within milliseconds can also be studied using fluorescence force microscopy, therefore allowing closer investigation of membrane dynamics and typically rapid events, such as the release of neurotransmitters.

How does fluorescence force microscopy work?

Fluorescence microscopy allows molecules tagged with a fluorescent label to be spotted via microscopy when the fluorophore is excited. However, current techniques do not enable tracking of a single molecule in the vertical dimension, thereby limiting the possible detection.

The addition of the atomic force microscopy method allows measurements of the evanescent wave’s distance-dependent intensity decay. The evanescent wave’s data can be used to modify a fluorescent particle’s vertical motions into displacement data of one nanometer or less. This displacement data shows how molecules move and have been applied to molecular dynamics in active cells.

The combination of atomic force microscopy with fluorescence microscopy allows detecting in the z plane, in addition to the x and y planes. This is possible due to high resolutions within the subnanometer range, over milliseconds. The evanescent wave is calibrated to the nanometer scale, which allows the fluorescent particle’s location to be detected in the z plane.

Applications of fluorescence force microscopy

Many applications focus on cellular interactions and membrane activity. Investigations of exocytosis in lung cells used the dual ability to detect activity and structural changes that fluorescence force microscopy possesses to look at exocytosis events and their effects on the cell.

They found that when lamellar bodies underwent exocytosis from the cell, the cell height and volume increased and then subsequently decreased back to normal levels. This provided the first evidence for the hypothesized dynamic nature of cells undergoing the slow exocytosis of lamellar bodies.

As a young field of microscopy, fluorescence force microscopy is continuously being explored and developed. Recently, developments have made it possible for force fluorescence microscopy to track single molecules.

This form of data collection is still being evaluated, as it relies on the accurate correlation of fluorescence and force microscopy, and the accuracy of the correlation can be influenced by a myriad of factors, such as drift and precision. Recent developments have further produced images with improved accuracy using correlative fluorescence and force microscopy, demonstrating that the technique holds promise for future applications.

Sources

Sarkar, A. et al. (2004). Simultaneous atomic force microscope and fluorescent measurements of protein unfolding using a calibrated evanescent wave. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.0403534101

Hecht, E. et al. (2012). Combined atomic force microscopy-fluorescence microscopy: analyzing exocytosis in alveolar type II cells. Analytical Chemistry. https://doi.org/10.1021/ac300775j

Frederick, W. et al. (2018). Orthogonal probing of single-molecule heterogeneity by correlative fluorescence and force microscopy. ACS Nano. https://doi.org/10.1021/acsnano.7b05405

Last Updated: Jan 17, 2020

Sara Ryding

Written by

Sara Ryding

Sara is a passionate life sciences writer who specializes in zoology and ornithology. She is currently completing a Ph.D. at Deakin University in Australia which focuses on how the beaks of birds change with global warming.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Ryding, Sara. (2020, January 17). What is Fluorescence Force Microscopy?. News-Medical. Retrieved on May 27, 2020 from https://www.news-medical.net/life-sciences/What-is-Fluorescence-Force-Microscopy.aspx.

  • MLA

    Ryding, Sara. "What is Fluorescence Force Microscopy?". News-Medical. 27 May 2020. <https://www.news-medical.net/life-sciences/What-is-Fluorescence-Force-Microscopy.aspx>.

  • Chicago

    Ryding, Sara. "What is Fluorescence Force Microscopy?". News-Medical. https://www.news-medical.net/life-sciences/What-is-Fluorescence-Force-Microscopy.aspx. (accessed May 27, 2020).

  • Harvard

    Ryding, Sara. 2020. What is Fluorescence Force Microscopy?. News-Medical, viewed 27 May 2020, https://www.news-medical.net/life-sciences/What-is-Fluorescence-Force-Microscopy.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
You might also like... ×
Scientist has ‘invisible enemy’ in sights with microscopic portraits of coronavirus