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Analytical and Preparative Chromatography

Chromatography (from the Greek words chroma, "color," and graphein, "to write") is a method for the separation of a mixture. In simple terms, the process involves dissolving the mixture in a fluid (mobile phase), which is then passed through another material (stationary phase).

11 Oct 2017

Analytical Chemistry Techniques

Analytical chemistry is a science in which materials are separated, identified and quantified. The techniques used in this branch of chemistry are defined as the chemical or physical principles employed to study a substance which is to be analyzed (the analyte).

11 Oct 2017

Analytical Chemistry Research

Analytical chemistry is an essential and fast-developing field of chemical research because it holds the key to the techniques and instruments used in the detection, isolation, characterization and purification of every chemical used in science.

11 Oct 2017

Epitranscriptome Analysis

The modifications in RNA sequence relating to the functional changes of a protein is known as the epitranscriptome. The protein synthesis involves two processes called transcription and translation. The change that occurs in RNA in the post-transcriptional stage results in alteration in function of the proteins.

20 Sep 2017

Using Mass Spectrometry for Protein Complex Analysis

Mass spectrometry (MS) is considered to be a powerful method for quickly and efficiently identifying protein samples. Most crucial issues in the analysis of protein complex through MS include specificity of protein complex and contaminants in background protein.

18 Sep 2017

MALDI Imaging

MALDI is the acronym for matrix-assisted laser desorption ionization. It is a type of mass spectrometry imaging that allows for label-free measurements of biological molecules such as proteins and peptides within tissue.

14 Sep 2017

Protein Structure Determination

Proteins play a crucial role in the world of research and their structures are, therefore, of great interest to the researcher. Many techniques and methods have been used to determine this, but one of the most important has been the discovery and development of X-ray crystallography.

11 Sep 2017

Microfluidic Sanger Sequencing Technologies

Sanger sequencing involves sequencing DNA by first copying single-stranded DNA by adding nucleotides to the chain. Each DNA fragment is terminated by the incorporation of a fluorescently labeled deoxynucleotide.

11 Sep 2017

Future DNA Sequencing Techniques

DNA sequencing has undergone radical advances since the first Sanger sequencing method was introduced in 1977.

11 Sep 2017

Illumina Sequencing

Next generation sequencing technologies were developed to improve on the speed and efficiency of shotgun-style Sanger sequencing. The technologies that fall into this category include Illumina (Solexa) sequencing, Roche 454 sequencing, Ion Torrent: Proton/PGM sequencing, and SOLiD sequencing.

6 Sep 2017

Krebs Cycle Overview

The Krebs cycle is named after its discoverer, Hans Krebs. It is also known as the citric acid cycle or the tricarboxylic acid cycle. It is a series of chemical reactions required for cellular respiration; it involves redox, dehydration, hydration, and decarboxylation reactions that produce ATP (adenosine triphosphate), a coenzyme energy carrier for cells. The waste product, in the form of carbon dioxide, is also produced as well as further sets of reactants used to regenerate the original reaction.

6 Sep 2017

Protein Complex Analysis

Proteins, also called polypeptides, are the polymers of amino acids. There are a total of twenty amino acids called monomers that exist naturally in proteins. Proteins are found in abundance and are differentiated from each other according to the number, type, and arrangement of amino acids in series, which comprise the mainstay of polypeptides.

6 Sep 2017

Protein Crystallization

Protein crystallization is an important tool to purify proteins as well as to demonstrate their chemical purity.

6 Sep 2017

Sanger Sequencing

The first DNA sequencing method was developed by Frederick Sanger in 1977. The technique was based on incorporation of chain-terminating dideoxynucleotides by DNA polymerase while replicating DNA.

5 Sep 2017

Shotgun Sequencing

Shotgun sequencing is the method used to sequence the human genome by Criag Venter at Celera Genomics.

5 Sep 2017

Raman Spectroscopy

Raman spectroscopy is a form of vibrational spectroscopy that is used to observe changes in a molecule; these may be rotational, vibrational or some other form.

5 Sep 2017

Fluorescence Spectroscopy

Modern instrumental chemical analysis employs a variety of techniques. Fluorescence spectroscopy is one of these methods and is based on the principle of excitation and emission of characteristic radiation by the analyte. This emission is then examined and the energies and relative intensities of the various parts of the spectrum tell the researcher both the types of molecules present and their concentration and absolute amount.

5 Sep 2017

Picosecond Absorption Spectroscopy

Absorption spectroscopy is one of the many techniques that utilize spectroscopic principles to study components of a mixture of substances. These are used to measure the absorption of radiation following interaction with a sample of the substance under study, which depends upon the frequency or wavelength of the radiated energy.

5 Sep 2017

Introduction to Optogenetics

Optogenetics is a technique used for the study of neural circuits in the brain. It is a branch of biotechnology that combines genetics and optical techniques to conceive and control a specific neural circuit in a living human brain.

31 Aug 2017

Metabolomics in Plants

Metabolomic studies in plants help to understand plant biology and physiology from the perspective of small chemical molecules. The role of metabolomics has found its way in plant biotechnology, transgenic research, improved tolerance to stress, and plant breeding.

29 Aug 2017