Life Science Articles

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Coupling and Ionization in Miniature Mass Spectrometry

Mass spectrometry (MS) is mainly involved in analysis of mass-to-charge ratio (m/z) and large quantity of ions traveling at high speed in vacuum. It is one of the most powerful tools used in investigation of the structure and composition of matter.

7 Dec 2017

History of Cell Reprogramming

Cell reprogramming is the process used for converting cells from one particular type to another. Over the past six decades, many techniques have been developed to define induced pluripotency.

7 Dec 2017

Epitranscriptome Sequencing Technologies

An epitranscriptome is a set of functionally appropriate RNA modifications. There are nearly one hundred known modifications of RNA; the most common modification in internal mRNA is N6-methyladenosine (m6A). Transfer RNAs (tRNAs), mRNAs, and ribosomal RNAs (rRNAs) are the three types of RNAs that are post-transcriptionally modified.

6 Dec 2017

Extending the Capabilities of TEM

Electron microscopes are tools of paramount importance for scientists in the field of life science and material science. Transmission electron microscopy (TEM) is a microscopy technique that uses electrons instead of light to produce detailed images of the structure of elements.

6 Dec 2017

Cryo-Electron Microscopy Techniques

The term “cryo-electron microscopy” (abbreviated as “cryo-EM”) is used in a large number of experimental methods. It works on the principle of imaging the radiation-sensitive samples by a transmission electron microscope (TEM) under low temperature conditions.

5 Dec 2017

Differential Scanning Calorimetry of Pharmaceuticals

Differential scanning calorimetry (DSC) is a technique which involves the sensitive measurement of the difference in the amount of heat required to increase the temperature of an analyte and that of a reference (such as an empty aluminium pan) as a function of temperature, whilst keeping both at almost the same temperature as each other.

5 Dec 2017

Countercurrent chromatography

Countercurrent chromatography (CCC) can be defined as "a technique of chromatographic separation based on the partition of solutes between two different liquid phases that does not get mixed together. The two phases are mobile phase and stationary phase (the support-free liquid)".

4 Dec 2017

Chiral Column Chromatography

Chiral column chromatography makes use of the concept of chirality. Chirality relates to study of the three-dimensional structure of molecules. The term comes from the Greek root chir-, hand, and means “handedness.” Chiral molecules show a left- and right-handed nature. They exist as mirror image forms that cannot be made to overlap byrotation. Molecules showing chirality rotate the plane of polarized light. The degree of rotation is called specific rotation or optical rotation.

30 Nov 2017

Challenges with Sanger Sequencing

Sanger sequencing is a method of sequencing DNA developed by Frederick Sanger in 1977. In Sanger sequencing, chain-terminating dideoxynucleotides are incorporated into the growing DNA chain at random positions. The sequence is then inferred from the set of incrementally terminated DNA chains separated by polyacrylamide gel electrophoresis. More recently, next generation sequencing (NGS) has begun to replace Sanger sequencing, primarily because it is able to sequence millions of DNA fragments simultaneously, compared to only 384 for Sanger sequencing.

30 Nov 2017

Protein and Peptide Microarray

Protein and peptide microarrays, the emerging tools for proteomics and clinical assays, are high-throughput methods that track binding events and activities, and decide the function of proteins on a large scale. Microarrays find their place in epitope mapping and serodiagnostic applications.

29 Nov 2017

Challenges of Protein Complex Analysis

Protein complex analysis involves extensive interpretation of the structure and function of proteins, which are present in complex biological samples. Due to this, techniques like protein complex analysis have great value in understanding the complex organisms. Though recent protein complex analysis methods are efficient in identifying the structure and of protein complex, there are some limiting factors.

29 Nov 2017

Challenges in Targeted Metabolomics

Targeted metabolomics is a quantitative approach of metabolomics as it depends on measuring the known metabolites of specific pathways. Since targeted metabolomics is a continually evolving field, it faces enormous obstacles in its widespread approach.

29 Nov 2017

Clinical Biophotonics

Biophotonics is a branch of science dealing with the interaction of light in biological substances such as tissues and cells at scales ranging from microns to the nano-level. It plays a vital role in the development of healthcare services by lowering the costs of treatment, with a suitable methodology for treatment of people in the aging society. Biophotonics consists of optics, photonics, nanotechnology, and biotechnology.

28 Nov 2017

FRET: Fluorophores

Fluorophore is the specific region or structural domain of a protein that exhibits fluorescence. In any fluorescence spectroscopy, fluorophores are the most significant part to image the protein–protein interactions.

10 Nov 2017

GFP Applications

In biochemistry and cell biology, the most highly studied and developed protein is green fluorescent protein (GFP), which is derived from jellyfish Aequorea victoria. GFP has a remarkable capacity to efficiently radiate visible internal fluorophore that is extremely valuable and naturally attractive. Innovative chances are created by the high-resolution crystal structure of GFP to decipher and control the association between structure and spectroscopic function of proteins.

10 Nov 2017

Biophotonics Techniques

Biophotonics is defined as the study of biological cells, tissues, and particles with the use of light in visible and near-visible range. It plays a major role in improving the imaging and therapeutic procedures that are commonly set for clinical purposes, including advances in lasers, optics, and fluorescence detectors. There are several primary techniques used in the biophotonic study.

15 Nov 2017

Biophotonics Research

Research in the field of biophotonics is driven by several research teams in universities, university hospitals, corporate research and analysis wings, and other such research institutions.

24 Nov 2017

Biophotonics in Cell Biology Studies

Biophotonics is a branch of science where the interaction between biological substances as well as light is used for probing, manipulating and imaging cells and tissues. This emerging technique is widely used in various fields such as medicine, agriculture, and in studies related to life science and environment.

24 Nov 2017

Bionanotechnology Applications

Bionanotechnology is a science that sits at the convergence of nanotechnology and biology. Nanobiology and nanobiotechnology are other names that are used interchangeably with bionanotechnology. The field applies the tools of nanotechnology to biological problems, creating specialized applications.

15 Nov 2017

Bioluminescence Resonance Energy Transfer (BRET)

Bioluminescence resonance energy transfer (BRET) is a noninvasive, advanced cell-based assay technology used for detection of protein–protein interactions. BRET involves fluorescent and bioluminescent protein tags with corresponding emission and excitation properties for investigation of resonance energy transfer between the protein tags in their vicinity owing to their interactions.

13 Nov 2017