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FRET: Fluorophores

Fluorophore is the specific region or structural domain of a protein that exhibits fluorescence. In any fluorescence spectroscopy, fluorophores are the most significant part to image the protein–protein interactions.

10 Nov 2017

GFP Applications

In biochemistry and cell biology, the most highly studied and developed protein is green fluorescent protein (GFP), which is derived from jellyfish Aequorea victoria. GFP has a remarkable capacity to efficiently radiate visible internal fluorophore that is extremely valuable and naturally attractive. Innovative chances are created by the high-resolution crystal structure of GFP to decipher and control the association between structure and spectroscopic function of proteins.

10 Nov 2017

Current and Future Applications of Optogenetics

Optogenetics is the science of using light to control the behavior of cells. It is one of the most rapidly evolving fields of applied research. Optogenetic techniques enable the control of electrically excitable cells such as muscle or nerve cells.

7 Nov 2017

GFP in Nature

The occurrence of bioluminescence is extremely diverse in nature. However, it is found to be rare if we calculate their presence across the entirety of the species. Although fluorescence and bioluminescence occur in aquatic and terrestrial organisms, they are widespread among aquatic organisms to attract mates, to find prey, or to protect themselves from their enemies.

6 Nov 2017

Citric Acid Cycle Regulation

The citric acid cycle is also known as the Krebs cycle or the tricarboxylic acid cycle. It is a series of reactions in a closed loop that are fundamental for cellular respiration. The citric acid cycle produces the high-energy molecule ATP (adenosine triphosphate) and byproducts that also form ATP in a further process called oxidative phosphorylation.

2 Nov 2017

Protein Purification Techniques

Protein engineering techniques are an essential part of customizing or producing proteins with specific properties which can be applied in various industrial processes. Thus they are crucial to biotechnological research.

24 Oct 2017

Nanoscience Advances in Biology

Nanoscience is the study of matter at molecular scales. That has been defined as manipulation of matter between the nanometer (nm) and micrometer (um) scale.

24 Oct 2017

Biophotonics Techniques

Biophotonics is defined as the study of biological cells, tissues, and particles with the use of light in visible and near-visible range. It plays a major role in improving the imaging and therapeutic procedures that are commonly set for clinical purposes, including advances in lasers, optics, and fluorescence detectors. There are several primary techniques used in the biophotonic study.

15 Nov 2017

Biophotonics Research

Research in the field of biophotonics is driven by several research teams in universities, university hospitals, corporate research and analysis wings, and other such research institutions.

24 Nov 2017

Biophotonics in Cell Biology Studies

Biophotonics is a branch of science where the interaction between biological substances as well as light is used for probing, manipulating and imaging cells and tissues. This emerging technique is widely used in various fields such as medicine, agriculture, and in studies related to life science and environment.

24 Nov 2017

Biophotonic Instruments

Biophotonics is the interdisciplinary science dealing with generation and utilization of light and radiant energy for detection, imaging, and manipulation of biological materials.

17 Oct 2017

Bionanotechnology Applications

Bionanotechnology is a science that sits at the convergence of nanotechnology and biology. Nanobiology and nanobiotechnology are other names that are used interchangeably with bionanotechnology. The field applies the tools of nanotechnology to biological problems, creating specialized applications.

15 Nov 2017

Biomedical Applications of Biophotonics

Biophotonics is the science of producing and utilizing photons or light to image, identify, and engineer biological materials. It is the integration of four major technologies: biotechnology, lasers, photonics, and nanotechnology.

17 Oct 2017

Bioluminescence Resonance Energy Transfer (BRET)

Bioluminescence resonance energy transfer (BRET) is a noninvasive, advanced cell-based assay technology used for detection of protein–protein interactions. BRET involves fluorescent and bioluminescent protein tags with corresponding emission and excitation properties for investigation of resonance energy transfer between the protein tags in their vicinity owing to their interactions.

13 Nov 2017

Bimolecular Fluorescence Complementation (BiFC)

Bimolecular fluorescence complementation (BiFC) is a recent technique used in the investigation and direct visualization of protein–protein interactions (PPIs) and interaction between proteins and other macro-molecules that are essential for survival of cells.

17 Oct 2017

BiFC Applications

In almost all cellular processes, there involves a complex protein network. Several high-throughput screening technologies are in use for discovering the enormous protein interaction networks.

17 Oct 2017

BiFC Limitations

Bimolecular fluorescence complementation (BiFC) assay is a nonintrusive fluorescence-based method used to exactly visualize the in vivo interaction of proteins with the help of fixed cells or live-cell imaging. This technique is also used to localize the subcellular interacting proteins without any additional external agents.

11 Oct 2017

BiFC Advantages

Interaction between proteins is the underlying mechanism for the creation of biological regulatory characteristics. The protein interaction study in live cells is essential as the interaction in a specific cell relies on proteins comprised in the cell and the external agents that stimulate the cell.

11 Oct 2017

Automation and Sample Preparation for Sanger Sequencing

Sanger sequencing is a DNA sequencing method developed by Fred Sanger in 1977. The technique is based on incorporation of chain-terminating dideoxynucleotides by DNA polymerase during replication of DNA. This creates DNA chain lengths corresponding to every nucleotide.

11 Oct 2017

Applications of Microfluidic Sanger Sequencing

Microfluidic Sanger sequencing is the implementation of the Sanger method of DNA sequencing on a chip; therefore, this allows for the manipulation of fluids at the submicron size. Requiring much smaller sample volumes, microfluidic Sanger sequencing automates the reaction, separation and detection processes.

11 Oct 2017