The mystery of Giglio island’s resistance to COVID-19

An intriguing new study explores the mechanisms by which an Italian island called Giglio remained resistant to coronavirus disease 2019 (COVID-19) even after much exposure. The paper, published on the preprint server medRxiv*, is an initial attempt to understand why some people do not contract the infection, with special reference to the role of endemic coronaviruses in eliciting cross-reactive immunity.

The researchers carried out an extensive seroprevalence study from April 29 to May 3, 2020, including the island’s three chief settlements. All adults living on the island were invited to participate in rapid serologic testing. A sample of saliva was obtained from all participants and validation testing by polymerase chain reaction testing for viral RNA. The participants were compared with the official municipal list of residents.

The locale

Giglio Island is a mountainous speck off the coast of Tuscany, Italy. At the time of the study, it was among the very few places where symptomatic COVID-19 had not yet manifested among the residents. Therefore, the scientists in the current study sought to detect antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel pathogen behind COVID-19), and to identify asymptomatic cases, if any. The study also aimed to initiate the investigation into the underlying mechanisms of resistance to the virus.

All the cases identified on this island so far have been imported cases. All such cases reached the island before social distancing was mandated, and all were then in the asymptomatic phase. Thus, their mingling with the local population led to a risk of significant infection.

In May 2019, the last census showed that there were just over 1,400 inhabitants, living mostly in three villages. The island has a population density of 59 square kilometers. However, since most of its inhabitants live in one of the three settlements, the actual population density is much higher, at ~2,000, ~3,50, and ~500 inhabitants per square kilometer in Giglio Porto, Giglio Castello, and Giglio Campese, respectively. When the smallest of the three settlements is excluded, the final estimated population density is ~2,500 per square kilometer. This makes it among the top 150 locations in Italy with respect to population density.

Despite these factors, favorable to a local outbreak, there was no single case of symptomatic COVID-19 among the local population.

The island is connected to the mainland via a ferry service that carries passengers twice a day. On average, only ten or so individuals are carried up and down each day. The island is therefore relatively isolated.

Isola del Giglio Porto Harbour. Image Credit: Can Daniel Amirak / Shutterstock
Isola del Giglio Porto Harbour. Image Credit: Can Daniel Amirak / Shutterstock

Study details

The investigators used a rapid antibody test that detected IgM and/or IgG, with a reported sensitivity of ~88% and a specificity of 100%. An independent study shows that while the test is 69% and 93% sensitive for IgM and IgG, respectively, it is almost 100% specific for both antibody classes.

Samples of saliva were tested by real-time transcriptase-polymerase chain reaction (RT-PCR). While some studies have shown saliva samples to have a reduced sensitivity compared to nasopharyngeal samples, other researchers disagree and suggest that these may be a good alternative, especially since they can be self-collected, providing a viable method of screening in low-resource or remote locations.

Screening results

The scientists screened 723 people, of which 634 were part of the 748 people residing on the island, while 89 were non-residents. The population screened comprised of 364 and 359 males and females, respectively. Among these, there were 185 cigarette smokers, using 14 cigarettes a day, on average. The median age was ~59 years overall, and ~49 years among the smoking population.

The rapid testing serology kit identified a single subject, previously undiagnosed, who was IgM positive, while correctly picking up IgG antibodies in two previously PCR-diagnosed patients. The newly identified subject was tested three times in all to confirm positivity. There were no positives among the saliva samples, though 66 of the 723 samples could not be used due to insufficient sample volume.

What are the implications?

The researchers postulate that the pristine air on the island might have played a dominant role in limiting the spread of the virus, in agreement with other studies showing a positive correlation between the lack of air pollution and the number of infections. The island's geographical, climatic and microenvironmental conditions must also be studied to elicit their potential contributions to this outcome.

Cross-reactive immunity may also play a role in this apparent resistance. It is possible that exposure to a closely related pathogen could have attenuated the severity of COVID-19 or prevented it altogether. The immunity might well have been mediated by a CD4 T cell clone reactive to SARS-CoV-2 antigens that are shared by those of endemic coronaviruses, which cause a common cold.

Such cross-reactivity is the subject of future research. The investigators also aim to carry out one more screening round of serology during the summer season, when thousands of tourists typically visit the island, to identify new cases and the routes of infection at that time.

*Important Notice

medRxiv publishes preliminary scientific reports that are not peer-reviewed and, therefore, should not be regarded as conclusive, guide clinical practice/health-related behavior, or treated as established information.


Journal reference:
Dr. Liji Thomas

Written by

Dr. Liji Thomas

Dr. Liji Thomas is an OB-GYN, who graduated from the Government Medical College, University of Calicut, Kerala, in 2001. Liji practiced as a full-time consultant in obstetrics/gynecology in a private hospital for a few years following her graduation. She has counseled hundreds of patients facing issues from pregnancy-related problems and infertility, and has been in charge of over 2,000 deliveries, striving always to achieve a normal delivery rather than operative.


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