Posted in | Cell Biology

Revealing Proteome Diversity with timsTOF: From Single-Cell Heterogeneity to Non-Canonical Biology

Webinar Date

  • 1 hour

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Precision medicine assumes an individual's proteome can point physicians toward the right diagnosis and treatment - but that idea rests on recognizing heterogeneity, and single-cell technologies are now revealing heterogeneity within a single cell type itself, reshaping how we understand organs like the heart. In this webinar, Jennifer Van Eyk and Nikolai Slavov explore how single-cell proteomics is uncovering biology invisible to bulk analysis. Van Eyk will share how single-cell proteomics of cardiomyocytes revealed unexpected subpopulations with distinct protein expression, unpredictable drug responsiveness, and cell-environment effects on disease-driving mutations, work now being integrated with spatial omics to map cardiac tissue architecture.

Slavov will present new mass spectrometry approaches revealing widespread alternate RNA decoding and its role in proteome diversity, alongside single-cell measurements of protein synthesis and degradation rates that expose general principles of protein abundance regulation. He'll also discuss hybrid fragmentation workflows that validate newly discovered protein sequences and modifications while supporting deeper proteome coverage.

Join the free webinar with certification of attendance.

Attend the Webinar to Learn: 

  • Understand how single-cell proteomics is uncovering previously hidden cellular heterogeneity
  • Explore new insights into cardiovascular biology and heart failure
  • Learn how advanced mass spectrometry technologies are expanding proteome discovery
  • Discover emerging approaches for measuring protein synthesis and degradation at the single-cell level
  • Gain insight into the future of precision medicine

This Webinar is Ideal For:

  • Proteomics, cell biology, and cardiovascular disease researchers
  • Mass spectrometry scientists and core facility directors
  • Cardiovascular and translational researchers
  • Drug discovery and pharmaceutical professionals
  • Systems biology and bioinformatics researchers
  • Clinical researchers and physician-scientists
  • PIs, postdocs, students, and lab managers in academia or industry

About the Webinar Speakers

 

Jennifer Van Eyk, PhD, is an international leader in the area of clinical proteomics and her lab has focused on developing technical pipelines for de novo discovery and larger scale quantitative mass spectrometry methods. This includes multiple reaction monitoring (MRM, also known as SRM) and most recently data independent acquisition. Dr. Van Eyk's laboratory is well known for the extreme technical quality of the data generated, rigorous quality control with tight %CV while applying these to key clinical questions. The aim is to maximize throughput and reproducibility in order to move targeted and robust discovery methods into large population healthy continuous assessment and clinical grade assays focusing on brain and cardiovascular diseases.

 

Nikolai Slavov is the Founding Director and CEO of Parallel Squared Technology Institute (PTI), and a Distinguished Professor at Northeastern University. The Slavov laboratory pioneered experimental and computational methods for single-cell proteomics and used them to connect protein covariation across single cells to functional phenotypes, including macrophage polarization, drug resistance priming, early mammalian development, stem cell differentiation and neurodegenerative diseases (Alzheimer’s and Parkinson’s disease). The proteomic technologies provided a foundation for establishing PTI. These results have been recognized by numerous awards, including NIH Director’s New Innovator Award, Allen Distinguished Investigator Award, HUPO Discovery in Proteomic Sciences Award, and a Biemann Medal by the ASMS.

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