The leading analytical tools in structural biology have traditionally been X-ray crystallography and liquid state NMR, but in recent years the role of solid state NMR has increased substantially through its ability to provide answers that neither liquids NMR nor X-ray can provide in a clear enough manner.
However, a serious problem that has limited the adoption of solid state NMR in structural biology has been the rather difficult setup of experiments, in both ensuring that they run efficiently, and also finally provide good data. Solid state NMR still requires trained spectroscopists in order to achieve great results, even in standard backbone assignment experiments, and even the most basic experiment can pose a challenge to hardware performance and instrument stability.
However, over the last decade the hardware has become increasingly stable and now delivers very high performance, achieving good reproducibility without the need for a trained rf-wizard to be present.
Over the same period, software development failed to keep pace with the advancements of hardware, but now a new dedicated software has closed that gap by providing an easy, interactive platform for conducting all basic solid state NMR experiments in structural biology.
Armed with only a basic knowledge of nuclear magnetic resonance basics, anyone can rely on the new software for easy set up of all backbone assignment and distance constraint experiments, making it ideal for the non-expert. And yet the expert also benefits, by an easy workflow that reduces the time to success by a factor of two, and reduces the probability of user error to virtually nothing.
Dr. Jochem Struppe, Product Manager Solid State NMR
Dr. Sebastian Wegner, Head of Solid State NMR Application
Who should attend?
Scientists working in structural biology including:
- Trained solid state NMR spectroscopists
- Liquid state NMR spectroscopist who consider doing solid state NMR
- Graduate students who do solid state NMR in structural biology
- Graduate students intending to do solid state NMR
- NMR spectroscopists intending to use solid state NMR
What you will discover?
You will be shown how to achieve not good, but great experimental results after a brief spectrometer and probe setup, aided and guided by TopSolids itself, and receive a comprehensive overview of the inner workings and structure of the software.
Using a research sample, we will show you how easy it is to automatically fine tune the spectrometer to focus on the sample of interest.
Effective use of TopSolids’ flow concept can achieve great results for your specific sample and we will show you how to reproducibly achieve the best results.
TopSolids consists of two basic parts. Part one, through an interactive flow bar, guides you through the setup of all necessary experimental parameters using a setup sample. Based on Part one’s results, Part two guides you through the parameter setup for the sample of interest.
A parameter manager keeps track of all important experimental parameters and you will learn how to exploit the parameter manager and the setup tools for great results.
A final phase sets up all the necessary n-dimensional experiments that can help you find a high fidelity structure for your biopolymer.