The SpectraMax® i3x Multi-Mode Microplate Reader is the ideal solution for research requirements. This adaptable instrument allows for the execution of a diverse array of critical cell-based assays, encompassing absorbance, fluorescence, and luminescence measurements.

Equipped with the SpectraMax MiniMax 300 Imaging Cytometer, leading SoftMax® Pro Software, and detection modules that can be configured by users, this exceptional reader permits the addition of optional detection modules at any point, thereby enhancing the reader’s functionality and facilitating the exploration of cellular pathways and protein expression using a single microplate reader.

SpectraMax i3x Multi-Mode Microplate Reader Unique Features. Video Credit: Molecular Devices UK Ltd

User-configurable applications

Detection modules that can be installed by users broaden the reader’s detection capabilities to encompass AlphaScreen, time-resolved fluorescence, TR-FRET (including HTRF), rapid kinetics with injectors, western blot detection, and additional functionalities.

Multi-channel functionality

The MiniMax 300 cytometer features two fluorescence detection channels, enabling the assessment of cell viability and toxicity assays, including viability assays and transfection efficiency evaluations.

Spectral optimization wizard

This integrated technology allows users to enhance their assay in a single step by swiftly determining the optimal excitation and emission wavelengths.

No-stain cell analysis

The StainFree Cell Detection Algorithm facilitates brightfield cell segmentation, enabling cell counting and confluency measurement without the use of destructive stains, thus streamlining the cell analysis process.

Cellular pathway analysis

The reader can capture cell confluence and viability imaging, western blot analysis, and quantification of nucleic acids and proteins, all in one device.

Auto intensity adjustment

The patented technology automatically collects data across a broad dynamic range, eliminating the necessity to re-read the plate due to saturation issues. PMT gain adjustments are rendered unnecessary through the use of high-power LEDs as part of the Spectral Fusion Illumination system.

Gold standard software

The highly regarded SoftMax Pro Software is acknowledged as the leading control and data analysis software for microplate readers. Designed to provide the simplicity, power, and flexibility needed for advanced data analysis, it features over 200 pre-configured protocols, robust data analysis tools, and a variety of output options.

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