What are Microbial Assays?

NewsGuard 100/100 Score

Introduction
Microbial Assays: An Overview
Microbial Assay Methods
Different Microbes Used in Microbial Assays
Culture Media Used
Summing Up
References


In pharmacological development and testing, many processes and techniques are employed to produce safe drugs for the worldwide market. This article will look at biological assays, a legal requirement in some countries.

Microbial Assays: An Overview

Microbial assays are a technique to assess a compound’s potency or concentration. This is carried out by exposing microorganisms to the compound and determining its effect on them. Microbial assays are required for antibiotic assay in the US Pharmacopoeia and the British Pharmacopoeia. This official publication lists medicinal drugs, their effects, and directions for their use.

Microbial assays provide information on how antibiotics inhibit microbial growth. The microbial assay is a comparative method. Other compounds whose activity can be studied with a microbial assay include amino acids and vitamins. Measured concentrations of the target compound are compared with the standard concentration of a particular compound with known activity, providing information on the compound under investigation.

Microbial assays are used for many purposes. These include antibiotic sterility testing, testing the mutagenicity or carcinogenicity of chemicals, disease diagnosis, and monitoring using immobilized enzymes. They are also used to evaluate the pharmacokinetic properties of drugs for human and animal use.

Microbial Assay

Image Credit: Mohammed_Al_Ali/Shutterstock.com

Microbial Assay Methods

There are several methods of performing a microbial assay. These include the disc diffusion method (otherwise known as the cylindrical cup plate method), the tube assay (or turbidimetric) method, the urease assay, and the luciferase assay. These methods have different uses.

The cylindrical cup plate method involves using circular plates of nutrient agar inoculated with a susceptible test organism. Some characteristics of the cup plate method include the selection of inoculant concentration to obtain suitable dose-response and sharp inhibition zones at different concentrations of the standard., keeping plates on a flat surface to ensure equal distribution of inoculant, and incubating plates at a suitable temperature over a set time.

Turbidimetric methods differ from the commonly used cylindrical cup plate assay by using tubes. Controls that are not inoculated with the target compound (usually antibiotics) are used to set the optical apparatus used to measure growth. When the tubes have been incubated, growth is arrested by adding formaldehyde R, or the opacity of tubes can be measured at a set incubation time. Appropriate statistical methods are used to calculate potency.

A urease assay is used to assay antibiotics that inhibit protein synthesis, as these antibiotics also inhibit urea production. The urease production is indicated by an increase in the media’s pH. These antibiotics include streptomycin, netilmicin, azithromycin, and erythromycin. Certain microbes such as Proteus mirabilis can be used as these produce the enzyme urease. Urease assays have limited application.

Like urease assays, luciferase assays also have limited application. This assay uses the enzyme luciferase to detect the presence of ATP in a microbial sample. This is detected by bioluminescence. The assay can detect ATP at low concentrations in a bacterial culture. Antibiotics such as aminoglycosides inhibit ATP production, and this assay can be used to analyze the potency of these drugs.

Another type of assay is the radioenzymatic assay, which uses a radiolabelling element to analyze the potency of compounds.

Different Microbes Used in Microbial Assays

There is no single microbe used in a microbial assay. The selection of a microbial strain depends on the antibiotic being selected for testing. These include Staphylococcus aureus (for amikacin, tylosin, and tobramycin), Bacillus pumilus (for chlortetracycline, spiramycin, and kanamycin sulfate), Staphylococcus epidermis (for antibiotics such as gentamicin, neomycin, and novobiocin) and Klebsiella pnumoniae (for streptomycin.)

Staphylococcus aureus

Image Credit: Kore/Shutterstock.com

Culture Media Used

Different culture media can be used in a microbial assay. The type of media selected depends on the microbe being used in the assay, for example, anaerobic bacteria, aerobic bacteria, or fungi. A fluid thioglycollate medium is used for anaerobic bacteria but can also be used for aerobic bacteria. Soyabean-casein digest is a culture medium for aerobic bacteria and fungi. Additionally, alternative thioglycolate medium can be used for viscous and turbid products as well as in devices that possess tubes with small lumina.

Culture media must be produced under strict conditions to ensure sterility so that the results obtained by the microbial assay on the potency of target compounds are error-free.

Summing Up

Microbial assays are crucial during research into the potency of drugs, chemicals, and other compounds. They are a requirement in numerous biomedical fields, and scientists can use several types of assays.

Different microbial strains are used in each assay to test the potency of drugs, and different culture media can be selected depending on the type of microbe used in the assay. Microbial assays are an important safety step in the process of drug and chemical research.

References:

Last Updated: Jan 31, 2023

Reginald Davey

Written by

Reginald Davey

Reg Davey is a freelance copywriter and editor based in Nottingham in the United Kingdom. Writing for AZoNetwork represents the coming together of various interests and fields he has been interested and involved in over the years, including Microbiology, Biomedical Sciences, and Environmental Science.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Davey, Reginald. (2023, January 31). What are Microbial Assays?. News-Medical. Retrieved on April 18, 2024 from https://www.news-medical.net/life-sciences/What-are-Microbial-Assays.aspx.

  • MLA

    Davey, Reginald. "What are Microbial Assays?". News-Medical. 18 April 2024. <https://www.news-medical.net/life-sciences/What-are-Microbial-Assays.aspx>.

  • Chicago

    Davey, Reginald. "What are Microbial Assays?". News-Medical. https://www.news-medical.net/life-sciences/What-are-Microbial-Assays.aspx. (accessed April 18, 2024).

  • Harvard

    Davey, Reginald. 2023. What are Microbial Assays?. News-Medical, viewed 18 April 2024, https://www.news-medical.net/life-sciences/What-are-Microbial-Assays.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Unraveling the mysteries of gastro-esophageal junction development