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Potential Contaminants in CBD and THC Oils

Cannabidiol (CBD) was initially discovered as a medication to treat Dravet syndrome, but is now used extensively to treat several medical and lifestyle disorders in addition to being used as recreational drug.

22 Jan 2019

What is Small-Angle X-Ray Scattering (SAXS)?

Small-angle X-ray scattering (SAXS) is an analytical technique to determine differences in density within a sample with a nanoscale resolution by analyzing the elastic scattering angles of X-rays.

22 Jan 2019

Metabolomic Profiling for Disease Diagnosis

Understanding the metabolic changes that occur in various disease states allows a more accurate diagnosis to be made.

22 Jan 2019

Virulent Bacteriophages and the Lytic Cycle

Lytic bacteriophage hijack the molecular machinery of a bacterial cell to make lots of progeny, and then burst, or lyse, the host cell.

22 Jan 2019

Uses of Short Hairpin RNA (shRNA) Interference

RNA interference refers to effective silencing or knocking down of a target gene by degrading its corresponding mRNA using short hairpin RNA (shRNA).

22 Jan 2019

Sequencing Antibodies

The investigation into the genetic makeup of antibodies is essential for antibody engineering, database banking, and the discovery of novel antibody clones.

22 Jan 2019

Micromanipulation and Microinjection Techniques

Microinjection refers to a technique wherein substances are injected into single cells using a very thin needle.

18 Jan 2019

Metabolic Changes in Aging Cells

Aging is caused by the deterioration of various cellular organelles and physiological processes.

18 Jan 2019

How Should Antibodies be Stored?

If stored incorrectly, the survival of the antibody can be dramatically reduced, lasting only a few weeks rather than a few years.

18 Jan 2019

How do Immunoassays Work?

There are various types of immunoassays, and these are described here.

18 Jan 2019

How to Present Flow Cytometry Data in a Publication

This article provides advice on presenting flow cytometry data in an academic publication, including presenting controls and standardizing the data.

17 Jan 2019

Fluorescence Quenching

Fluorescence quenching is a physicochemical process that lowers the intensity of emitted light from fluorescent molecules.

17 Jan 2019

Eosinophil Function

This article provides a brief overview of eosinophils; specialized cells of the immune system involved in anti-parasitic and inflammatory processes.

17 Jan 2019

Electrochemistry Mass Spectrometry (EC-MS) Applications

EC-MS can be used to analyze a wide range of biological reactions, including redox reactions and protein structures.

17 Jan 2019

Applications of Parallel Accumulation and Serial Fragmentation (PASEF)

PASEF traps precursor ions eluted during liquid chromatography in a tunnel, where they are accumulated and ordered depending on their collisional cross-sectional area and charge.

17 Jan 2019

Why are Beta-Actin and GADPH Used as Controls in Western Blotting?

Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments.

14 Jan 2019

Transfer-PCR (tPCR) Steps

tPCR involves three steps; PCR amplification, an intermediate step which creates megaprimers, and the introduction of the gene to the destination vector.

14 Jan 2019

The Tau (CDK5) Signaling Pathway

In Alzheimer’s disease, CDK5 phosphorylates Tau, leading to the accumulation of tau within the axons of nerve cells and eventually, apoptosis.

14 Jan 2019

Affinity Chromatography vs. Protein A/G Purification

Affinity chromatography and protein A/G purification are antibody purification methods to remove ligands from extracts. They have different applications.

14 Jan 2019