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Electrochemistry Mass Spectrometry (EC-MS) Applications

EC-MS can be used to analyze a wide range of biological reactions, including redox reactions and protein structures.

17 Jan 2019

Applications of Parallel Accumulation and Serial Fragmentation (PASEF)

PASEF traps precursor ions eluted during liquid chromatography in a tunnel, where they are accumulated and ordered depending on their collisional cross-sectional area and charge.

17 Jan 2019

Why are Beta-Actin and GADPH Used as Controls in Western Blotting?

Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments.

14 Jan 2019

Transfer-PCR (tPCR) Steps

tPCR involves three steps; PCR amplification, an intermediate step which creates megaprimers, and the introduction of the gene to the destination vector.

14 Jan 2019

The Tau (CDK5) Signaling Pathway

In Alzheimer’s disease, CDK5 phosphorylates Tau, leading to the accumulation of tau within the axons of nerve cells and eventually, apoptosis.

14 Jan 2019

Affinity Chromatography vs. Protein A/G Purification

Affinity chromatography and protein A/G purification are antibody purification methods to remove ligands from extracts. They have different applications.

14 Jan 2019

What are Bispecific Monoclonal Antibodies?

Bispecific antibodies are comprised of two monoclonal antibodies held together by a flexible peptide linker.

10 Jan 2019

AI in Forensic Science

This article describes the areas of forensic science that are changing due to artificial intelligence (AI) and machine learning.

8 Jan 2019

What is the CLARITY Technique?

CLARITY is a method used for the analysis of proteins and nucleic acid location within the brain.

9 Jan 2019

Protein A/G Purification for Antibodies

The protein A/G purification method is used to purify monoclonal IgA and IgG antibodies using a recombinant of Protein A and Protein G.

9 Jan 2019

History of Electrochemistry

Electrochemistry refers to the study of the chemical processes that are responsible for the movement of electrons which, in turn, causes electricity.

9 Jan 2019

Transfer-PCR (tPCR) Applications

In tPCR, target DNA is amplified and integrated into a destination vector in a single tube, without the need to purify the intermediate PCR product.

9 Jan 2019

How are Antibodies Validated?

Antibody validation ensures that antibodies are selective, reproducible, and specific enough for their application.

8 Jan 2019

HEK293 Cells: Applications and Advantages

Human embryonic kidney 293 (HEK293) cells are one of the most common cell lines used for research purposes. This article examines why they are so popular.

8 Jan 2019

CLARITY Methodology

The CLARITY technique is a method used to clear tissues of lipids and enable immunolabelling of whole, intact organs.

8 Jan 2019

Amyloid Cascade Hypothesis

The amyloid cascade hypothesis postulates that the initial event which triggers neuronal degradation in Alzheimer’s disease is enhanced amyloid-β generation and aggregation.

8 Jan 2019

Computational-based Approaches to Protein Function Prediction

The most effective way of determining protein structure from sequence alone is by using computational methods.

7 Jan 2019

Autophagy Regulation

Too much or too little autophagy has been linked to a number of diseases. It is therefore important that we understand the regulators of this process.

7 Jan 2019

Phage Display: Applications in Research

Phage display is an analytical technique used to study protein-protein interactions and the relationship between a protein and the encoding DNA.

7 Jan 2019

PBMCs: Applications in Research

PBMCs are currently being used for various research applications; including regenerative medicine, CAR-T cell therapy, and most recently, biomarker discovery.

7 Jan 2019