GC-MS Analysis of CBD and THC

NewsGuard 100/100 Score

There has been a sharp rise in the use of cannabis-containing products in the past few years. This is mostly due to the legalization of medical and recreational cannabis use in several US states. Components of cannabis include tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN).  One method by which cannabis is analyzed is through GC-MS.

Cannabis farm - Ryland zweifeRyland zweife | Shutterstock

Why is there a need to analyse CBD and THC?

Cannabis is one of the most widely used drugs in the world and the availability of various concentrations of the ingredients of cannabis has made the production process complex. It has also been noted that one of the main components of cannabis, THC, has increased in concentration in the cannabis plant over time.

Although cannabis is supposed to have low toxicity, recent studies show that cannabis and its ingredients may potentially be a risk factor for psychotic symptoms. Thus, there is a need to analytically examine the concentrations of its active ingredients from different places and in time.

What methods are used for gas chromatography-mass spectrometry?


The various solvents used to process cannabis include n-Hexane, acetaldehyde, chloroform, vanillin, hydrochloric acid, alcohol, ethyl ether, methanol, acetone, and ethyl acetate. All these reagents are of analytical grade and can be readily purchased.

Extraction of compounds

In one study, CBD and THC extraction was performed by soaking 25 g of cannabis plant in 500 mL of n-hexane for 10 days. The mixture was then sonicated and the solvent was evaporated so that the sample could be analyzed.

Sample preparation

The process of gas chromatography-mass spectrometry (GC-MS) requires extensive sample preparation. To do so, 500 µL of solution are transferred into a 2 mL gas chromatography vial. The vial can be heated in a bath for 12 minutes to evaporate and decarboxylate the solvent. The residue is then dissolved in 1.5 mL ethanol and shaken before subjecting to gas chromatography analysis.


Processing is accomplished through the combination of gas chromatography and mass spectrometry. A capillary tube is used in gas chromatography, a process in which the column dimensions (length, diameter, and thickness) and the phase properties are defining. The differing affinities of molecules for the stationary phase of the column allows for separation of the molecules as they travel along the gas chromatograph.

Based on their properties, they elute off the column at different times and are thus analyzed by the mass spectrometer at different times. The mass spectrometer captures these molecules, then ionizes, accelerates, deflects, and detects different molecules based on their mass to charge ratio.

Used together, mass spectrometry and gas chromatography allow for the identification of a compound at a higher resolution than analyzing the sample individually on either of these methods.

What conditions are needed to perform the analysis?

The column size is 15 m x 0.25 mm, and the phase is 5% diphenyl and 95% dimethylpolysiloxane. Although different types of analytical columns can be used to separate cannabinoids, columns with small diameters, thin films, and non-polar stationary phases are most commonly used. However, non-polar columns lack sufficient polarity to separate cannabidiol and cannabichromene. In such cases, an intermediate polarity column, such as 35% diphenyl with 65% dimethyl silicone, may be used.

The vector is a hydrogen atom, and the temperature is kept at 280°C. The cannabinoids can be separated in less than 3 minutes using a short and small-diameter column. However, a wide-bore column with a diameter of 530 µm is sometimes preferred due to higher sample capacity; the elution rate with such columns would be approximately 9 minutes.

Further Reading

Last Updated: Jan 25, 2019

Dr. Surat P

Written by

Dr. Surat P

Dr. Surat graduated with a Ph.D. in Cell Biology and Mechanobiology from the Tata Institute of Fundamental Research (Mumbai, India) in 2016. Prior to her Ph.D., Surat studied for a Bachelor of Science (B.Sc.) degree in Zoology, during which she was the recipient of an Indian Academy of Sciences Summer Fellowship to study the proteins involved in AIDs. She produces feature articles on a wide range of topics, such as medical ethics, data manipulation, pseudoscience and superstition, education, and human evolution. She is passionate about science communication and writes articles covering all areas of the life sciences.  


Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    P, Surat. (2019, January 25). GC-MS Analysis of CBD and THC. News-Medical. Retrieved on May 23, 2024 from https://www.news-medical.net/life-sciences/GC-MS-Analysis-of-CBD-and-THC.aspx.

  • MLA

    P, Surat. "GC-MS Analysis of CBD and THC". News-Medical. 23 May 2024. <https://www.news-medical.net/life-sciences/GC-MS-Analysis-of-CBD-and-THC.aspx>.

  • Chicago

    P, Surat. "GC-MS Analysis of CBD and THC". News-Medical. https://www.news-medical.net/life-sciences/GC-MS-Analysis-of-CBD-and-THC.aspx. (accessed May 23, 2024).

  • Harvard

    P, Surat. 2019. GC-MS Analysis of CBD and THC. News-Medical, viewed 23 May 2024, https://www.news-medical.net/life-sciences/GC-MS-Analysis-of-CBD-and-THC.aspx.


The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.