Life Science White Papers

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Life Science White Papers are in-depth articles aimed to educate and inform site visitors interested in life science research. Some of these papers are derived from sources where we have existing commercial relationships. Such articles are clearly labelled as sponsored content and are only accepted when the content is deemed to add value to the core editorial ethos of News Medical.

Application of ASTRA 6 Software Tool for Analysis of Drug-Antibody Components

Antibody-drug conjugates (ADCs) are a new type of drug that are currently the subject of much interest due to their potential application in the targeted delivery of cancer treatments. One of the key factors in the effective design of ADCs is the Drug Antibody Ratio (DAR).

From Waters | Wyatt Technology 2 Aug 2014

Nanoparticle and Macromolecular Analysis using the DynaPro DLS Plate Reader

In immunoglobulin biopharmaceuticals, aggregation is a common issue that occurs during all stages of drug development. Since a large number of monoclonal antibody titers are produced in bioreactors, effective aggregate removal techniques are required in manufacturing processes.

From Waters | Wyatt Technology 2 Aug 2014

Characterization of Liposome Size and Encapsulation using FFF-MALS-QELS

Liposomes are synthetic vesicles composed of a lipid bilayer surrounding an aqueous core in which drugs can be contained. These vesicles therefore provide a valuable mechanism for drug delivery in the fields of clinical therapy and research. It is important to monitor liposome size and encapsulation during the research, manufacture, and quality control of these important vesicles.

From Waters | Wyatt Technology 1 Aug 2014

Characterization of Hemoglobin using SEC-MALS Technology

Hemoglobin is a major protein component of mammalian red blood cells that plays a vital role in transporting oxygen from the lungs around the body. Following the release of oxygen, carbon dioxide attaches to the hemoglobin and is then removed from the body.

From Waters | Wyatt Technology 1 Aug 2014

Application of CG-MALS for Analyzing Metacomplex Formation in the Hetero-Association of Multivalent and Bivalent Binding Partners

For standard techniques in the analysis of macro-molecular interactions, such as ELISA and SPR, a binding partner is required to immobilize the molecule on a surface for the measurement of the binding affinity.

From Waters | Wyatt Technology 1 Aug 2014

Characterization of Protein Aggregate Composition using Light Scattering Techniques

Protein aggregation refers to the clumping together or accumulation of mis-folded proteins. This aggregation can occur during the production, administration, storage, and shipping of therapeutic protein compounds, which can compromise their stability, efficacy, and safety.

From Waters | Wyatt Technology 1 Aug 2014

Measuring Intrinsic Protein Fluorescence with Modular Spectroscopy Tools

Protein structure and function can be accurately studied using intrinsic fluorescence measurements. A better understanding about the activity or conformational states of proteins under various temperatures, pH, and ion concentrations can be achieved by estimating the quantity of fluorescence.

From Ocean Insight 1 Aug 2014

Characterization of Monomers, Dimers and Hexamers of Insulin using MALS and Column Chromatography (SEC/GPC)

Generally, in protein and peptide analyses, knowing a molecule’s oligomeric state is useful in determining whether the protein exists in its biologically active form. To this end, the first step usually involves separating and characterizing the molecular species using size exclusion chromatography (SEC).

From Waters | Wyatt Technology 31 Jul 2014

Application of Composition-gradient Multi-angle Light Scattering for Measuring the Self-Association of Insulin

Composition-gradient multi-angle light scattering (CG-MALS) is a biophysical technique that can be used to measure the self-association of macromolecules in solution. This technique allows fast, reproducible, and label-free measurement of this self-association.

From Waters | Wyatt Technology 31 Jul 2014

Using Molecular Weight and Net Charge to Discriminate Contaminated and Unmodified Heparin Lots

Heparin is an injectable anticoagulant that has been used for medical purposes for more than seven decades. However, this agent has come under considerable scrutiny after certain pharmaceutical lots have been found to contain chondroitin sulfate. Therefore, advanced characterization techniques are needed to accurately determine the exact chemical composition of heparin lots.

From Waters | Wyatt Technology 31 Jul 2014

nanoXIM•CarePaste Reduces Dental Hypersensitivity and Promotes Enamel Remineralization

Dental hypersensitivity is a major, common dental condition that occurs in approximately 57% of adults. Among individuals with periodontal disease, the prevalence can reach as much as 98%

From Fluidinova 8 May 2014

Using the Luna-FL Dual Fluorescence Cell Counter for Quantification of GFP Transfection Efficiency

Transfection is a process whereby foreign genetic material is introduced into eukaryotic cells. To this end, several transfection techniques have been developed. However, irrespective of the method used, the transfection efficiency is not 100%.

From Logos Biosystems 30 Apr 2014

Automated Fluorescence Cell Counters for Cell Counting and Viability Analysis

From the late 1990s, a range of image-based automated cell counters have been launched that provide precise data on cell number and viability. These counters essentially use a combination of image analysis software and light microscopy. A digital camera captures magnified cell images and software algorithms are used to precisely detect cells and distinguish them from non-cellular objects such as cell debris.

From Logos Biosystems 30 Apr 2014

Using the LUNA-FL Dual Fluorescence Cell Counter for Rapid Cell Counting and Viability Measurement of Yeast Cells

Yeast is a very important organism in genetics and cell biology research. Together with its unicellularity, its small genome size and low number of genes make it an invaluable modelling system, particularly for the study of cell cycle regulation and signal transduction.

From Logos Biosystems 30 Apr 2014

APOLLO Raman Microspectrometer for Pharmaceutical Monitoring

Pharmaceutical monitoring is an integral part of the production, testing and manufacturing process.

From CRAIC Technologies, Inc. 3 Apr 2014

Solid-state ChIP Platform for High Throughput Analysis of Gene Regulation

In recent years, epigenetics has become an increasingly important focus in the worlds of academia and industry as researchers try to better understand the fundamental s of transcription regulation. Developments in high throughput ChIP together with the use of automated liquid handing and next generation sequencing is laying the foundations for analysis of global gene expression and understanding the epigenetic landscape in a huge number of cell types across a variety of diseases and treatments.

From Chromatrap® - Porvair Sciences Ltd 19 Mar 2014

Chromatrap 96 High Throughput ChIP for Screening Multiple Epigenetic Targets

Advancements in the field of epigenetics are rapidly growing as researchers elucidate the principles controlling gene regulation via the in vivo binding of proteins to DNA. A major aspect in this is the need to study a vast number of regulatory mechanisms to map interactions between DNA and protein following disease and pathogen stimulus.

From Chromatrap® - Porvair Sciences Ltd 17 Mar 2014

Chromatrap Immunoprecipitation Assay Enriches Epigenetic Histone Methylation Marks from Primary Biopsy Samples

The uterus is a pear-shaped organ that consists of a lower barrel shaped cervix and a large upper body with the 'fundus' over the uterine tubes. It has a thick muscular wall, which houses the human fetus from conception to birth.

From Chromatrap® - Porvair Sciences Ltd 17 Mar 2014

Chromatrap® Technology for Studying Complex Epigenetic Mechanisms

Chromatin immunoprecipitation (ChIP) is a widely used technique for mapping the interactions between intracellular proteins and their DNA targets in gene regulation. The epigenetic mechanisms that affect chromatin formation and control the availability of the DNA backbone to transcription factor regulators is an important area of research. ChIP is a technology that allows researchers a glimpse into the regulatory mechanisms that gene expression.

From Chromatrap® - Porvair Sciences Ltd 17 Mar 2014

Setting Effective Product Specifications in Pharmaceutical Manufacturing

An important aspect of Quality by Design (QbD) is to establish meaningful and realistic specifications. Well-defined specifications control the performance of products, as they derive from associations between clinical behaviour and the variables determined routinely during processing for quality control. This article investigates the process of setting specifications, taking particle size as an example which is a major parameter for a variety of pharmaceutical formulations.

From Malvern Panalytical 17 Mar 2014

Life Science White Papers

Application of ASTRA 6 Software Tool for Analysis of Drug-Antibody Components

Nanoparticle and Macromolecular Analysis using the DynaPro DLS Plate Reader

Characterization of Liposome Size and Encapsulation using FFF-MALS-QELS

Characterization of Hemoglobin using SEC-MALS Technology

Application of CG-MALS for Analyzing Metacomplex Formation in the Hetero-Association of Multivalent and Bivalent Binding Partners

Characterization of Protein Aggregate Composition using Light Scattering Techniques

Measuring Intrinsic Protein Fluorescence with Modular Spectroscopy Tools

Characterization of Monomers, Dimers and Hexamers of Insulin using MALS and Column Chromatography (SEC/GPC)

Application of Composition-gradient Multi-angle Light Scattering for Measuring the Self-Association of Insulin

Using Molecular Weight and Net Charge to Discriminate Contaminated and Unmodified Heparin Lots

nanoXIM•CarePaste Reduces Dental Hypersensitivity and Promotes Enamel Remineralization

Using the Luna-FL Dual Fluorescence Cell Counter for Quantification of GFP Transfection Efficiency

Automated Fluorescence Cell Counters for Cell Counting and Viability Analysis

Using the LUNA-FL Dual Fluorescence Cell Counter for Rapid Cell Counting and Viability Measurement of Yeast Cells

APOLLO Raman Microspectrometer for Pharmaceutical Monitoring

Solid-state ChIP Platform for High Throughput Analysis of Gene Regulation

Chromatrap 96 High Throughput ChIP for Screening Multiple Epigenetic Targets

Chromatrap Immunoprecipitation Assay Enriches Epigenetic Histone Methylation Marks from Primary Biopsy Samples

Chromatrap® Technology for Studying Complex Epigenetic Mechanisms

Setting Effective Product Specifications in Pharmaceutical Manufacturing

Fluidics in Modern Healthcare & Medical Device Innovation

Scaling 3D biology: Automation, imaging, and the future of high-throughput research

How does norepinephrine influence anxiety-related behaviours?

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