Amity Manning, assistant professor of biology and biotechnology, has received a three-year, $300,000 award from the Smith Family Awards Program for Excellence in Biomedical Research. The grant will support her research on the molecular biology of cancer cells, work that may lead to a better understanding of how tumors evolve and how some become resistant to chemotherapy drugs--knowledge that could pave the way for new kinds of anti-cancer medications.
The awards program was established in 1991 by the Richard and Susan Smith Family Foundation, which works to effect permanent change in the lives of residents of Greater Boston. The program helps launch the careers of newly independent faculty members in Massachusetts whose research focuses on biomedicine, with the aim of achieving medical breakthroughs in such areas as cancer, heart disease, diabetes, and neuroscience. Manning is one of just six recipients of the prestigious award this year.
In her research, Manning explores the biology of cancer cells, looking in particular at molecules that help regulate changes in the genome, or genetic material, within these cells. It is known that certain mutations and other defects in the genome are responsible for the uncontrolled cell division that occurs in cancer, for the ability of tumors to adapt to changes in their environment and to metastasize, and for the resistance that some cancers develop to anti-cancer medications. But there are a host of unanswered question about how those genetic changes occur and the effect they have on cancer cells.
Manning's current focus is a molecule known as retinoblastoma protein, or pRB. In normal tissue, pRB helps determine when cells can divide. In tumor cells, the protein is either defective or not present, which leads to uncontrolled cell division. In previous work, funded by the National Institutes of Health, Manning and her research team showed that pRB also plays a role in assuring that the chromosomes are correctly segregated when a cell divides.
During normal cell division, or mitosis, a human cell's 46 chromosomes are first duplicated, then the pairs of duplicates line up in the center of the cell. Thin fibers called microtubules emerge from structures known as centromeres and pull the duplicate sets of chromosomes apart so two new "daughter" cells, each with an identical set of chromosomes, can be formed.
In the absence of normally functioning pRB, the segregation process goes awry. As a result, the daughter cells can end up with the wrong number of chromosomes (known as aneuploidy). In her research, Manning has found that the absence of pRB is associated with defects in chromatin, the complex of proteins and genetic material that make up chromosomes. These defects may alter the way microtubules latch onto and move the chromosomes.
Errors in chromosome segregation, known as chromosome instability, are associated with genetic differences that are seen between tumors of the same type, and even between cells within the same tumor. They may also contribute to the increasing number of mutations that can occur as a tumor grows. The resulting genetic heterogeneity is believed to account for the fact that a tumor that responds well to chemotherapy may later become resistant to the anticancer drugs.
"We believe chromosome instability plays a role in the heterogeneity we see within tumors," Manning says, "and that heterogeneity is linked to the ability to acquire drug resistance. Because the cells within a tumor are genetically different, a drug that kills one of the daughters of a cell division may not be sufficient to kill the other. And because cancer cells proliferate so quickly, a tumor will quickly generate more and more copies of the resistant cell. It's a moving target."
With the funding from the Smith Foundation, Manning hopes to learn more about how pRB helps regulate chromosome segregation in normal cells to better understand what doesn't happen or what happens differently when pRB not functional. This knowledge may help identify targets for new medications that could prevent segregation errors and assure that the chromosomes separate accurately. "Since all progeny will then be identical," Manning says, "all the cells of the tumor may remain similarly sensitive to therapy and drug resistance will be limited."
Manning said she believes her research will show that pRB plays a multifaceted role in regulating chromosome segregation, one that likely involves recruiting proteins that regulate the organization of DNA. Changes in the activity of such regulators are known as epigenetic changes; they can impact what genes are expressed and how chromatin behaves, without altering the DNA sequences. "We believe that pRB functions as a sort of scaffold," Manning said. "It brings the other regulators to the genome and those regulators determine how the chromatin of organized. So when pRB is not there, those regulators are not recruited the way they should be.
"We want to look at which of those regulators might be altered and if there are specific regions of the genome that are most sensitive to changes in those alterations when pRB is absent. And then, hopefully, if we can identify those changes, we can try to understand, functionally, how they are involved in cell division."
Manning said her team will be looking closely at changes that affect the centromere. "The centromere is the functional region of the chromosome that is important for attachments to microtubules and proper segregation. We think pRB is particularly important in recruiting regulators to the centromere, such that the absence of pRB may cause defects in centromere structure, which would alter how microtubules attach and lead to errors in chromosome segregation."
Unlike mutations and other genetic alterations, these epigenetic changes are reversible, Manning said. "If we can show that the absence of pRB results in changes in the way that chromatin structure is regulated, we may also be able to show how those changes can be reversed. To me, that is one of the most exciting aspects of this work."