Discovery heralds a new strategy in the hunt for antibiotics

Scientists have identified a key process in the way bacteria protect themselves from attack - and it heralds a new strategy in the hunt for antibiotics.

The researchers from the University of Leeds have pieced together how bacteria build their outer, defensive wall - in essence, the cell's armor plating.

The research has focused on the gram-negative bacteria Escherichia coli, but the process they have discovered is shared by many pathogenic gram-negative bacteria - so it could have importance for tackling other gram-negative pathogens, including the top three on the World Health Organisation's list of priority pathogens.

The findings are published today (01/05) in the journal Nature Communications.

Our findings are changing the way we think about the way these cells constantly renew and replenish the proteins that make up the outer membrane.

Understanding that process of how bacteria build their cell wall in greater detail may identify ways we could intervene and disrupt it.

In doing so, we can either destroy the bacteria altogether or reduce the rate at which they divide and grow, making bacterial infections less severe.

We are at the start of a quest that could result in new, drug-based therapies that work either alone or with existing antibiotics to target these disease-causing bacteria."

Dr. Antonio Calabrese, University Academic Fellow in the Astbury Centre for Structural Molecular Biology

The research has focused on the role of a protein called SurA. Known as a chaperone, the job of SurA is to martial other proteins from where they are made, at the center of the cell, to where they are needed, in this case to bolster the bacterium's outer wall.

Proteins are long chains of amino acids that must adopt a defined structural shape in order to function effectively. Without the chaperone SurA, the essential proteins needed to build the cell wall run the risk of losing their structural integrity on their journey to the outer membrane.

Using advanced analytical techniques, the scientists mapped how the chaperone SurA recognizes proteins to transport them to the bacterial outer membrane.

Dr. Calabrese said: "For the first time we have been able to see the mechanism by which the chaperone, SurA, helps to transport proteins to the bacterial outer membrane. In effect it does this by cradling the proteins, to ensure their safe passage. Without SurA, the delivery pipeline is broken and the wall cannot be built correctly."

Professor Sheena Radford, FRS, Director of the Astbury Centre for Structural Molecular Biology said "This is an exciting discovery in our quest to find weak spots in a bacteria's armory that we can target to stop bacterial growth in its tracks and build much-needed new antibiotics.

"It's early days, but we now know how SurA works and how it binds its protein clients. The next step will be to develop molecules that interrupt this process, which can be used to destroy pathogenic bacteria."

It was only through the work of a great team from across the Astbury Centre that we were able to finally understand how SurA shuttles proteins to the bacterial outer membrane."

Dr. David Brockwell, Associate Professor in the Astbury Centre for Structural Molecular Biology

The research was funded by the UK Biotechnology and Biological Sciences Research Council and used equipment funded by the BBSRC and Wellcome Trust.

Source:
Journal reference:

Calabrese, A.N., et al. (2020) Inter-domain dynamics in the chaperone SurA and multi-site binding to its outer membrane protein clients. Nature Communications. doi.org/10.1038/s41467-020-15702-1.

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