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Broad Clinical Labs developed and launched the blended genome exome (BGE) method, a high-throughput DNA library construction assay that produces low-pass whole-genome (1–4× mean depth) and deep whole-exome (30–40× mean depth) data in a single sequencing run. BGE is cost-effective as it leverages miniaturized reactions for high scale 384-sample processing batches, empowers most genomic discoveries possible with deep whole-exome sequencing and captures global common single-nucleotide polymorphism diversity. The BGE process relies on several automation instruments to ensure quality control of this process at scale, including the Lunatic UV/Vis spectrophotometer from Unchained Labs.
The Lunatic is primarily utilized at three major quality control steps: gDNA stock concentration assessment upon entry to the Broad system, gDNA stock concentration and quality assessment upon plating and target normalization into the BGE process, PCR Plus library yield and quality assessment prior to exome capture. Samples flow simultaneously through the process resulting in a usage level of 12 Lunatic plates per lab batch. In this webinar, we will explore the BGE product through the lab workflow and highlight the implementation of the Lunatic for reliable, scalable quality control.
Join the free webinar with certification of attendance now.
Join the Webinar to Learn:
- Learn how the blended genome exome (BGE) method delivers low-pass whole-genome and deep whole-exome data in a single sequencing run
- Discover how leveraging automation with miniaturized reactions and 384-sample batches make high-throughput library prep more cost-effective
- See how Lunatic provides quick and accurate dye-free quant and QC across the BGE workflow
This Webinar is Ideal For:
- Genomics and Sequencing Scientists working with whole-genome or whole-exome methods
- Laboratory Automation and Workflow Specialists focused on high-throughput sample processing
- Quality Control and Assay Development Professionals in genomic or molecular labs
- Core Facility Managers and Directors overseeing large-scale sequencing operations
- Research Scientists and Principal Investigators in population or clinical genomics
- Instrument and Platform Specialists interested in UV/Vis spectrophotometry for QC applications
About the Webinar Speakers
Reagan is a Clinical Laboratory Lead at the Broad Institute of MIT and Harvard, where she supports laboratory operations, process development, and workflow optimization across genomics and clinical laboratory capabilities. With more than six years of experience in next-generation sequencing and laboratory automation, she has worked extensively to develop, scale, and optimize high-throughput sequencing workflows using a range of automated liquid handling and quantification technologies. Faye's academic background includes a B.S. in Biology from the University of Massachusetts Amherst, with a focus in genetics and neuroscience.
Crystal Emery is the Product Manager for Lunatic at Unchained Labs. She holds a B.S. in Aquatic Biology from UC Santa Barbara and a Master’s in Marine Science from San Francisco State University. Previously, she worked with next-generation sequencing (NGS) workflows at Corteva Agriscience and supported semiautomated laboratory technologies at Isolation Bio. She enjoys collaborating with researchers to streamline complex workflows and deliver innovative instrumentation solutions that drive efficiency in the lab.