Nunchuck represents a groundbreaking platform, uniting two essential stages essential for creating precisely sized Lipid Nanoparticles (LNPs): low-volume screening and efficient scaled-up LNP formulation. In a mere 10 minutes, users can swiftly evaluate eight different total flow rates (TFRs) to identify the optimal conditions.
Users can seamlessly transition to producing LNPs ranging from 0.250 mL to 1 L, all on the same day and using the same consumable. This eliminates the need for time-consuming one-flow-rate-at-a-time processes, providing users with the ultimate one-and-done LNP manufacturing solution.
- 0.250 mL—1 L formulations
- Screen 8 TFRs in 10 min
Capabilities
The nun gets it done
To make LNPs using Nunchuck, simply load the syringes filled with aqueous and organic solutions into the Nunchuck, lock them into the Nun consumable, and start the process. Nunchuck’s novel microfluidic mixing chambers can handle flow rates from 0.5 to 30 mL/min, so users can create a wide range of LNP sizes. As the Nun consumable is reusable, users can use it multiple times on the same day with the same chemistry.
Image Credit: Unchained Labs
White belts welcome
Nunchuck is incredibly user-friendly, allowing even a novice to initiate a run within minutes. Start by selecting either a screening or preparation run, then provide the necessary information about the formulation. The final step involves choosing the flow rate ratio, total flow rates, and the desired sample volume. In just five short minutes, users can become proficient LNP operators.
Image Credit: Unchained Labs
Hit the target
The choice of LNPs varies based on the target, and adjusting the total flow rate (TFR) is a crucial, yet often time-consuming step in achieving the desired size. Nunchuck streamlines this process with a dedicated screening run, efficiently determining the TFR within just 10 minutes. Users can screen up to eight total flow rates with remarkable consistency, simplifying the quest for ideal conditions without any hassle.
Image Credit: Unchained Labs
Level up
Regardless of the volume needed, Nunchuck consistently provides high-quality LNPs, from 0.250 mL to 1 L. Eliminate the need to use different instruments for sample optimization and large-scale LNP formulation development.
Image Credit: Unchained Labs