Using SNPs and STRs for Human Identification

Verogen offers software to simultaneously sequence several forensic SNPs and STRs in a single test at the same time.

Simplified STR & SNP Analysis

A high number of forensic laboratories presently use PCR and capillary electrophoresis (CE)-based techniques to investigate short tandem repeat (STR) markers and alternative polymorphisms.

Sequencing STRs allows an extra degree of resolution for identifying the variations between individuals, such as the ability to observe intra-STR single nucleotide polymorphisms, or SNPs.

SNPs can be utilized for identification when DNA is too degraded for STR analysis, or to deliver biogeographical and physical estimation for investigative intelligence.

Image Credit: Verogen, Inc.

Benefits of SNP & STR Analysis with NGS

  • Alleles of identical sizes, but different sequences can be resolved through STR sequencing.
  • Several polymorphisms can be analyzed at once using high throughput sequencing.
  • Different types of STR can be investigated simultaneously, with no requirement for the re-testing of SNPs to deliver investigative intelligence

From DNA to Identification

When human identification is critical, a technology that yields effective investigative intelligence from a DNA sample. Verogen delivers a host of capabilities specific to the MiSeq FGx Forensic Genomics Solution, such as:

  • Forensic genetic genealogy—Broaden the network of information to find an individual
  • Mitochondrial DNA sequencing—Acquire additional answers from difficult samples
  • STRs and SNPs sequencing—Convert samples into physical characteristics

This overview describes how these exclusive capabilities optimize the analysis of DNA, from producing a profile to discovering human identification.

STR and SNP Sequencing Workflow

Verogen provides a complete, fully validated DNA-to-data solution created for forensic genomics. Forensically relevant SNPs and STRs are PCR amplified at the same time, with indexed adapters included in every amplicon in an individual round of PCR.

The ensuing generation of DNA clusters and sequencing by synthesis is carried out using the MiSeq FGx Instrument. The sequencing data can finally be analyzed and interpreted off-line utilizing the ForenSeq Universal Analysis Software.

Sample and Library Preparation

DNA Extraction & Quantitation

The current DNA extraction and quantitation techniques are recommended for use.

Library Preparation

ForenSeq DNA Signature Prep Kit (for nuclear DNA) Assay around 200 genetic markers in one test. All reagents to construct sequencing libraries from forensic DNA samples are included.

Sequencing

MiSeq FGx Instrument

The first completely validated next-generation sequencing system for forensic genomics.

MiSeq FGx Reagent Kit

Ready-to-use, pre-filled reagent cartridges, exclusively created for the MiSeq FGx System.

Data Analysis

ForenSeq Universal Analysis Software

Complete functionality to investigate and interpret a broad range of database samples and forensic casework.

Uses of SNP and STR Analysis

Criminal Casework

Targeted sequencing of STR and SNP loci that are forensically relevant enables the production of leads, even on cold cases. Learn More »

Image Credit: Verogen, Inc.

Forensic DNA Databases

NGS can assist laboratories in constructing high-quality forensic profiles for national DNA databases, with a more efficient turnaround. Learn More »

Image Credit: Verogen, Inc.

Identifying Missing Persons

NGS provides a single workflow and platform to analyze a broad range of complex samples found in missing person cases. Learn More »

Image Credit: Verogen, Inc.

Disaster Victim Identification

The genetic analysis of mass fatality evidence with NGS allows the user to acquire the greatest amount of information possible from samples that have been highly compromised. Learn More »

Image Credit: Verogen, Inc.

Additional Resources

Overview: NGS for Forensic Genomics

Read an overview of NGS and how it can be applied in forensic genomics.

Image Credit: Verogen, Inc.

Take a Genomic Leap Forward in Forensic Science

The MiSeq FGx System is the first completely validated NGS solution created specifically for forensic science.

Image Credit: Verogen, Inc.

Harnessing NGS to Solve Crimes

Criminologists utilize MiSeq FGx for high-resolution DNA profiling to construct optimized forensic DNA databases.

Image Credit: Verogen, Inc.

Recreating a Face Based on DNA

Researchers determine genetic variants that shape facial characteristics to allow facial imaging from DNA.

Image Credit: Verogen, Inc.

About Verogen, Inc.

Introducing the world’s first sequencing company solely dedicated to forensic science. Forensic laboratories are unique, and require unique scientific solutions.

We get this. Because we live it. We create thoughtfully tailored genomic solutions for forensic DNA labs. The kinds of tools we longed for when we worked in those labs ourselves. We formed an independent company with dedicated resources because we believe it’s the best way to fully address those unique needs.

The last few decades have seen tremendous advancements in molecular biology techniques, such as massively parallel sequencing. While our colleagues in other scientific fields have embraced and benefited from these improvements, forensic genomics has yet to realize the potential of the evolved technology. It’s time we had modern tools tailored to our needs.

Verogen understands this. Our sole focus is to advance science to help unlock the true potential of forensic genomics. Powered by Illumina technology and free of legacy method allegiance, we are uniquely positioned to support forensic labs with innovative solutions purpose-built for the challenges of DNA identification. Working in partnership with the community, we can elevate the forensic genomics lab’s role in preserving public safety—and improve global justice for all.


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Last updated: Feb 12, 2020 at 5:06 AM

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