In vitro assessment of ocular toxicity and phenotypic rescue strategies

A client wanted to assess the retinal toxicity of two lead compounds and determine how each impacts the retina.

In vitro assessment of ocular toxicity and phenotypic rescue strategies

Image Credit: Newcells Biotech

Solution

Step 1:

Newcells’ experts worked with the client to understand the problem

The client required a retina model to quickly assess the retinal toxicity of two compounds and identify any differences. Newcells recommended studying how each chemical impacts cell viability and cellular composition in its iPSC-derived retinal organoid model.

Step 2:

Newcells created a study protocol and design that efficiently provided data of value

The experiment investigated compound-induced toxicity on mature, healthy retinal organoids differentiated for 150 days and exposed for 72 hours.

The readouts included retinal organoid viability assays and immunofluorescence with six important retinal markers to assess organoid cell composition (ganglion cells, photoreceptors, amacrine cells, horizontal cells, and Muller glia).

Step 3:

From initiation to data in less than two months

The study comprised the experimental phase, data processing and analysis, and a data summary presentation.

DATASET: Four compounds, one positive control, a hundred retinal organoids, seven immunofluorescence staining per compound, ATP depletion, and LDH release for each compound.

The study was finished in just under two months.

Step 4:

Newcells delivered a detailed report with a reliable dataset

Newcells provided a strong dataset that included a large number of immunofluorescence images.

Outcomes for the client

The study confirmed one of the compounds’ retinal toxicity and provided the client with a deeper understanding of how the compound impacts various cell types.

About Newcells Biotech

Newcells Biotech develops in vitro cell-based assays for drug and chemical discovery and development.

Using our expertise in induced pluripotent stem cells (iPSCs), cellular physiology, and organoid technology, we build models that incorporate the “best biology” for predicting in vivo behavior of new drugs.

Our experts have developed and launched assays to measure transporter function, safety, and efficacy in a range of cell and tissue types, including kidney, retina and lungs.

We have the capability to develop and implement protocols to measure cilia beat frequency and toxicity on small airway epithelial cells model, retinal toxicity and disease modelling on retinal organoids and retina epithelium, as well as drug transport in the kidney, DDI and nephrotoxicity across human and a range of preclinical species.


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Last updated: Nov 25, 2024 at 5:08 AM

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