Phlebotomy - Drawing of Blood

Phlebotomy is the drawing of blood. A phlebotomist is someone with special training to do so. However, in many countries, other healthcare professionals can also draw blood (e.g., doctors, nurses, laboratory, and emergency medical technicians).

The phlebotomist aims to obtain blood samples, which is most commonly done via venous system through venipuncture, which is one of the most common invasive procedures used in modern medicine.

Phlebotomy may be employed for diagnostic, therapeutic, or charitable reasons, such as infections, polycythemia vera (a neoplasm caused by overproduction of red blood cells from the bone marrow), blood transfusions, respectively.

Image Credit: Cmhompoo Suriyo / Shutterstock
Image Credit: Cmhompoo Suriyo / Shutterstock

Blood drawing procedure

The most appropriate site to draw blood is selected based on vessel accessibility, patient age, and health status. Usually, the antecubital area, where the elbow bends, is used to access the median cubital vein. Since it’s easy to visualize and puncture, it’s also more stable than its neighbors, the cephalic vein, accessory cephalic vein, and basilic vein.

A tourniquet is applied about a hand’s width above the selected site, the patient is asked to make a fist, and the needle is positioned at a 30-degree angle while entering the vessel swiftly. The tourniquet is then removed once sufficient blood has been acquired, and the needle is withdrawn gently from the vessel while pressure is applied at the puncture site for a few minutes to control bleeding.

In patients who are more difficult to obtain a median cubital vein blood sample from (e.g., infants), other sites such as the heel or scalp may be used in combination with a local anesthetic, such as lidocaine, to reduce pain.

Phlebotomy: Venipuncture Procedure

Safety while taking blood

To ensure the patient and healthcare professional's safety and maintain the quality of the sample, proper identification of the patient and labeled collection tubes are important. Appropriate hand hygiene (i.e., thoroughly washing hands or using an alcohol hand rub), sterile gloves and equipment should be used. The entry site should be disinfected with 70% alcohol before puncturing the blood vessel.

Other safety measures include avoiding recapping of used needles and a designated puncture-resistant bin for the disposal of all sharp objects contaminated with the patient’s blood. These important measures attempt to reduce the likelihood of needle-stick accidents, which can spread disease and be quite distressing for those involved.

Indications and contraindications for drawing blood

To treat a patient, diagnosing infectious agents or other abnormalities is the first step, to which blood samples are a vital component. In addition to diagnosing, the therapeutic value of access to blood vessels includes infusions for applying pharmaceuticals such as those required for nutritional purposes or chemotherapy.

The major contraindications for phlebotomy are skin conditions like cellulitis or abscesses that would cause direct seeding of infectious agents (e.g., bacteria) into the blood. Other complications that should be taken into account are the presence of vascular access devices in hospitalized patients, vascular grafts, bleeding under the skin, and if the patient has palpable venous fibrosis.

Further Reading

Last Updated: Mar 1, 2021

Dr. Damien Jonas Wilson

Written by

Dr. Damien Jonas Wilson

Dr. Damien Jonas Wilson is a medical doctor from St. Martin in the Caribbean. He was awarded his Medical Degree (MD) from the University of Zagreb Teaching Hospital. His training in general medicine and surgery compliments his degree in biomolecular engineering (BASc.Eng.) from Utrecht, the Netherlands. During this degree, he completed a dissertation in the field of oncology at the Harvard Medical School/ Massachusetts General Hospital. Dr. Wilson currently works in the UK as a medical practitioner.


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