Life Science Articles

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The Baculovirus Expression Vector System (BEVS)

The Baculovirus Expression Vector System (BEVS) is used to produce recombinant proteins. The BEVS has applications in virology and biotechnology.

25 Oct 2018

Dye-ligand Affinity Chromatography for Protein Purification

Synthetic dyes can act as alternative ligands for proteins and can be used as part of a protein purification protocol for affinity chromatography.

25 Oct 2018

Improving Chromatography Quality and Efficiency

This article explains the equations used to calculate chromatography efficiency and how to improve both efficiency and quality of the resolution.

25 Oct 2018

Nanocomposites in Biomedicine

Nanocomposites range between 1‑–100 nm in diameter and are up of ceramic, metal, or polymer. It is imbued with nanoscale fibers or particles to increase catalytic activity, alter physical properties of the material, adjust the refractive index and optical properties, or create superparamagnetic materials.

24 Oct 2018

What are Spheroplasts?

Spheroplasts are cells (fungal, plant or bacterial) with partial cell wall. They can be found naturally and in the laboratory.

24 Oct 2018

What is Holotomography?

Holotomography is used to image a microscopic label-free three-dimensional object in real time, such as tissues or cells using a laser.

24 Oct 2018

Wide-field Fluorescence Microscopy

Wide-field fluorescence microscopy is a widely applied imaging technique used to examine cells and investigate their internal structures.

24 Oct 2018

Holotomography in Cell Biology

Holotomography is a scientific method used to image cells in two- and three-dimensions without the need for sectioning or labeling with external proteins.

24 Oct 2018

Minimizing Photobleaching in Fluorescence Microscopy

Photobleaching is a process whereby fluorophores lose their vibrancy, causing the signal to fade. Read our step-by-step guide to reducing photobleaching.

24 Oct 2018

Mannan-binding Lectin Serine Protease (MASP) Family

The Mannan-binding lectin serine protease (MASP) protein family is critical for the complement pathway in the immune system.

24 Oct 2018

What are PARP inhibitors?

PARP inhibitors are pharmaceutical drugs that can be used to treat many different forms of cancer, including ovarian and breast cancer.

24 Oct 2018

Cell Preparation for Flow Cytometry

This article describes how to prepare cells for flow cytometry, a popular technique used to analyze cell size, volume, and molecule expression.

23 Oct 2018

Cell Cycle Analysis Using Flow Cytometry

This article describes how to analyze the cell cycle using flow cytometry, with examples from scientific studies.

23 Oct 2018

Ion Channel Electrophysiology

Ion channels are imperative to the normal functioning of the cell. The study of ion channels is called electrophysiology, and is described in this article.

23 Oct 2018

Patch Clamp Configurations

The patch clamp technique is used to study ion channel electrophysiology across the cell membrane. There are several configurations, each with pros and cons.

23 Oct 2018