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  • DNA Methyltransferase Enzymes: DNMT1 to DNMT3
    Life Sciences - 20 Jul 2023
    Global cytosine methylation patterns in mammals appear to be controlled by a complex interaction of DNA methyltransferases: DNMT1, DNMT2, DNMT3A and DNMT3B.
  • The Polymerase Chain Reaction
    Life Sciences - 20 Jul 2023
    Polymerase chain reaction (PCR) is a revolutionary laboratory technique that enables the replication of a specific DNA sequence. Using PCR, millions of copies of a target DNA can be easily synthesized...
  • History of Polymerase Chain Reaction (PCR)
    Life Sciences - 20 Jul 2023
    The development of the polymerase chain reaction (PCR) has been a major breakthrough in the scientific world. Over time, the technique has evolved beyond the confines of its simple initial design and...
  • Automation and Sample Preparation for Sanger Sequencing
    Life Sciences - 20 Jul 2023
    Sanger sequencing is a DNA sequencing method developed by Fred Sanger in 1977. The technique is based on incorporation of chain-terminating dideoxynucleotides by DNA polymerase during replication of...
  • Challenges of Protein Complex Analysis
    Life Sciences - 19 Jul 2023
    Protein complex analysis involves extensive interpretation of the structure and function of proteins, which are present in complex biological samples. Due to this, techniques like protein complex...
  • Coupling and Ionization in Miniature Mass Spectrometry
    Life Sciences - 19 Jul 2023
    Mass spectrometry (MS) is mainly involved in analysis of mass-to-charge ratio (m/z) and large quantity of ions traveling at high speed in vacuum. It is one of the most powerful tools used in...
  • Optimizing Protein Purification with FPLC
    Life Sciences - 19 Jul 2023
    Fast protein liquid chromatography (FPLC) is a liquid chromatography technique for separation of protein molecules under pressure. It differs from high performance liquid chromatography in that the...
  • Life Science Applications of Countercurrent Chromatography
    Life Sciences - 19 Jul 2023
    Developments in spectroscopic analysis and routine availability of X-ray crystallography have simplified structural elucidation of natural compounds. However, isolating bioactive components of...
  • Label-free Protein Microarray Techniques
    Life Sciences - 19 Jul 2023
    Advances in material science combined with nanofabrication and computational design have led to the discovery of label-free protein microarray techniques. These techniques are of enormous benefit for...
  • Suzuki-Miyaura Cross-Coupling Reaction
    Life Sciences - 19 Jul 2023
    The Suzuki-Miyaura reaction was first published in 1979 and is a non-toxic method of producing biaryls, alkenes and styrenes for organic chemistry.

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