Scarab Genomics releases new E. Coli strain

ScarabXpress is the latest strain in the multiple-deletion series (MDS) of Scarab Genomics Clean Genome® E. coli. Based on the MDS™42 strain, ScarabXpress T7lac contains a chromosomal copy of the T7 RNA polymerase gene expressed from a unique, tightly controlled lac promoter.

MDS™42 is a K-12 strain with a 15% genome reduction to remove non-essential genes, sequences for recombinogenic and mobile DNA, and cryptic virulence genes, while preserving robust growth and protein production. Scarab has removed Insertion sequence (IS) elements, which are a prevalent, but frequently overlooked, problem that contributes to clonal instability. In addition, Clean Genome® strains lack prophage, which can be stress-activated and cause premature lysis and failed runs during fermentation. Genome reduction optimizes Clean Genome E. coli for recombinant DNA and protein production, providing enhanced genetic stability and improved metabolic efficiency, and making them the strains of choice for applications ranging from routine cloning to manufacturing biotherapeutics.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Chan Zuckerberg initiative joins Scale Bio's ‘100 Million Cell Challenge’ to accelerate single cell genomics research