Olympus launches objective lenses, enabling super-deep imaging of tissues, live-cell imaging and light sheet microscopy techniques

Dedicated to multiphoton excitation microscopy, Olympus introduces its XLPLN10XSVMP and XLSLPLN25XGMP objective lenses with an 8 mm working distance and support for a large range of refractive indices (RI). The objectives enable super-deep imaging of tissues treated with the latest clearing agents as well as live-cell imaging and light sheet microscopy techniques.

Compatible with the industry leading FVMPE-RS and FV1200MPE multiphoton microscopy systems and easily adaptable to home-build light sheet microscopes, the new objectives boast a range of features providing important benefits to researchers. Both objectives offer super-long working distances of 8 mm, providing the capability and space for structural and live-cell imaging deep within large intact samples (such as whole mouse brain), especially useful in neuroscience applications. A high transmittance range of 400-1600 nm extending into the infra-red spectrum allows deeper imaging with minimal damage to the tissue, and is well-suited to combine conventional multicolour MPE imaging with label-free methods like third- and second-harmonic generation. A field number of 27.5 provides excellent scattered light collecting abilities and a correction collar allows compensation of refractive index mismatches. Furthermore, spherical aberrations are corrected across the visible to infra-red (IR) range, with additional chromatic aberration correction in the IR range.

The XPLN10XSVMP is also extremely versatile with multi-immersion capability enabling its use with water, silicone and oil as well as clearing agents such as SCALEVIEW(1), Clarity(2) and SeeDB (RI 1.33-1.52)(3). Deep macro observation is achieved with high resolution at a large field of view (FOV – 1.8 mm at 10X magnification). The XLSLPLN25XGMP also supports the latest in clearing agents such as Clarity and SeeDB for cutting-edge research techniques, aided by a high RI immersion capability of 1.41-1.52 (SeeDB requires an RI of 1.49).

These additions to Olympus’ XLPLN series of objectives extend the company’s lead in the field of multiphoton excitation microscopy. The objectives enable the interrogation of large intact samples (such as whole-brain high-resolution imaging) and structural and molecular analysis of large assembled biological systems. The large FOV provided by the objectives enhances data collection from samples treated with the latest clearing agents by several orders of magnitude and this combined with unsurpassed flexibility and correctional capabilities makes them the clear choice for researchers using MPE or light sheet microscopy techniques.

For further information please visit www.olympus-europa.com/microscopy.

References

  1. Tomer R, Li Y, Hsueh B, Deisseroth K, Nature Protocols 9 (7), 1682-1697 (2014 - published online 19 June 2014); doi:10.1038/nprot.2014.123
  2. Chung K, Deisseroth K, Nature Methods 10, 508-513 (2013); doi 10.1038/nmeth.2481
  3. Ke MT, Fujimoto S, Imai T, Nature Neuroscience 6 (8), 1154-1161 (2013); doi: 10.1038/nn.3447

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Evident Corporation - Life Sciences. (2019, April 29). Olympus launches objective lenses, enabling super-deep imaging of tissues, live-cell imaging and light sheet microscopy techniques. News-Medical. Retrieved on December 02, 2024 from https://www.news-medical.net/news/20140911/Olympus-launches-objective-lenses-enabling-super-deep-imaging-of-tissues-live-cell-imaging-and-light-sheet-microscopy-techniques.aspx.

  • MLA

    Evident Corporation - Life Sciences. "Olympus launches objective lenses, enabling super-deep imaging of tissues, live-cell imaging and light sheet microscopy techniques". News-Medical. 02 December 2024. <https://www.news-medical.net/news/20140911/Olympus-launches-objective-lenses-enabling-super-deep-imaging-of-tissues-live-cell-imaging-and-light-sheet-microscopy-techniques.aspx>.

  • Chicago

    Evident Corporation - Life Sciences. "Olympus launches objective lenses, enabling super-deep imaging of tissues, live-cell imaging and light sheet microscopy techniques". News-Medical. https://www.news-medical.net/news/20140911/Olympus-launches-objective-lenses-enabling-super-deep-imaging-of-tissues-live-cell-imaging-and-light-sheet-microscopy-techniques.aspx. (accessed December 02, 2024).

  • Harvard

    Evident Corporation - Life Sciences. 2019. Olympus launches objective lenses, enabling super-deep imaging of tissues, live-cell imaging and light sheet microscopy techniques. News-Medical, viewed 02 December 2024, https://www.news-medical.net/news/20140911/Olympus-launches-objective-lenses-enabling-super-deep-imaging-of-tissues-live-cell-imaging-and-light-sheet-microscopy-techniques.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
The Art of Science: EVIDENT Launches Expanded Global Image of the Year Contest