Agena Bioscience releases LungFUSION Panel to identify gene fusions in NSCLC tumors

NewsGuard 100/100 Score

Agena Bioscience today released the LungFUSION Panel for rapid and sensitive identification of oncogenic ALK, RET, and ROS1 gene fusions in non-small cell lung cancer tumors.

Using as little as 10 ng of cDNA template, the research use only, single-well panel identifies expressed translocations of known clinical relevance in FFPE and fresh frozen tissue samples. The MassARRAY-based assay provides a simpler, sensitive alternative to conventional screening methods such as IHC stains and FISH, both of which require high-quality samples and time consuming protocols.

A similar NSCLC panel evaluating ROS1 and RET translocations was published in February by investigators at Wayne State University School of Medicine in the Journal of Thoracic Oncology. The investigators reported their custom MassARRAY-based test -; which uses a different assay design than Agena Bioscience's new panel -; as capable of "detecting an event in test specimens containing 0.5% positive tumors" and having "exceptionally low sample input, high cost efficiency, flexibility, and rapid turnover."

"Rare events in NSCLC tumor samples can be difficult or laborious to determine through optical genetic analysis techniques. The LungFUSION panel is an inexpensive, single-well assay amenable to formalin-fixed tissue and fine needle aspirates which may be of varying quality, allowing NSCLC studies to expand in sample size and confidence level within existing research budgets," said Marisa Pearce, Agena Bioscience's Sr. Director, Marketing.

The new LungFUSION panel contains built-in controls to simultaneously assess RNA quality, report DNA contamination, and determine the relative amount of lung tissue present in the sample. Agena Bioscience's software automatically reports translocation status for each sample as well as a confidence score.

The MassARRAY system detects genetic variation directly by end-point PCR and label-free mass spectrometry. Due to the high confidence level mass spectrometry provides, MassARRAY data is frequently used to validate variants detected from genome sequencing in research and clinical settings. The platform's flexible nature and simple bioinformatics allows laboratories to develop targeted, actionable panels for routine or specialized assays for as little as tens of dollars per test.


The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Novel combination therapy shows promise in difficult-to-treat endometrial cancer, study finds