JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, works closely with users at the University of Sheffield where their NanoWizard® AFM systems are being used to further understand soft matter and biological systems at the molecular scale in the Hobbs SPM Group in the Department of Physics.
Dr Nic Mullin adjusts a sample on the JPK NanoWizard® 3 AFM system at the University of Sheffield
Dr Nic Mullin is a senior experimental officer in the SPM Group of Professor Jamie Hobbs in the Department of Physics & Astronomy at the University of Sheffield. His research is based around instrument development for scanning probe microscopy for the study of soft matter and biological systems at the molecular scale.
As well as applying existing methods and instrumentation, the group have the added challenge to develop new equipment and protocols to allow the study of different samples at higher magnifications applying new detection systems. This has been formalised (for biological systems) in the Imagine: Imaging Life initiative, which brings together electron microscopy, super-resolution optical microscopy and AFM to study living systems. One of the major goals is to use AFM to provide information that complements the information garnered from the electron and super-resolution optical microscopies.
The Group has many years of experience using AFM. For example, having the resolution to see not just where molecules are and how they are arranged, but what their conformation is, is vital for many of the things they are interested in. Being able to resolve features with sufficient signal to noise ratio in real space is also a huge plus and means that they are not restricted to looking at systems that are static in time or spatially ordered.
Also, the ability to make mechanical measurements, either as a contrast mechanism, or as an indication of structure/function is really useful. The ability to do all this under relevant conditions and without too many restrictions on sample preparation makes AFM a fantastic tool for the research of the Group. It makes selection of the appropriate systems vital in the quest of the Group to retain a position of leadership in the field. Choosing JPK has proved to be a successful choice.
Dr Mullin takes up the story about their use of JPK AFMs and their integration with the other microscopies. “We work extensively with collaborators using transmission electron microscopy and super-resolution optical microscopies, often using these techniques to obtain complementary information. We use one of our JPK systems for correlative AFM-STORM. The raw performance of our JPK NanoWizard® systems is exceptional and the user interface is intuitive and well designed. The integration with optical microscopy is extremely good. the system was clearly designed to work with biological samples in liquid. This really shows.
For example, it's really straightforward to keep everything clean and free from contamination. The other big benefit is JPK themselves – we are interested in developing and optimizing our experiments, and JPK really support us with that, providing tweaks, workarounds, hacks and information that really allow us to expand our capabilities and the kind of things we can study – for this reason people from JPK are often acknowledged in our papers.”