Discovery points toward a new strategy for treating Parkinson's disease

University of Utah Health scientists have identified a molecule that slows cells' production of alpha-synuclein, a protein that forms toxic aggregates in the brains of people with Parkinson's disease. Its discovery points toward a new strategy for treating the disease and potentially stopping its progression.

Researchers led by Associate Professor of Neurology Daniel Scoles, Ph.D., reported on the molecule, known as A-443654 in the Journal of Biological Chemistry.

More than 10 million people worldwide live with Parkinson's disease, a neurodegenerative disorder that causes problems with movement and balance. These symptoms, which worsen as the disease progresses, are due to the loss of dopamine-producing neurons in the brain. Medications that mimic dopamine or boost the neurotransmitter's levels in the brain can help control symptoms. But existing therapies do not eliminate the disease or stop its progression.

As researchers seek better options for patients, many have their sights set on alpha-synuclein. In healthy brains, alpha-synuclein is thought to help nerve cells communicate. But in patients with Parkinson's disease and certain other neurodegenerative disorders, including Lewy body dementia and multiple system atrophy, alpha-synuclein molecules stick to one another and form abnormal aggregates inside neurons. As the protein coalesces into slender fibrils and larger aggregates, it interferes with the cells' health and, scientists suspect, eventually kills them.

While the exact cause of neuron death in Parkinson's disease is not known, alpha-synuclein seems to be a significant instigator.

Most cases of Parkinson's disease are characterized by an overabundance of alpha-synuclein. The prevailing thought is that if you lower its overall abundance, this would be therapeutic."

Daniel Scoles, Ph.D., Associate Professor of Neurology

Scoles and colleagues hoped to achieve this by finding a way to rein in cells' production of the potentially toxic protein. Duong Huynh, Ph.D., a Research Associate Professor at Department of Neurology in the Department of Neurology, devised a novel way of conducting the search.

Using gene editing tools, he inserted a gene that encodes a light-producing protein from fireflies into lab-grown human cells, positioning it where it would be turned on whenever the alpha-synuclein gene was active. Under the right growing conditions, the human cells glow when the alpha-synuclein gene is on. When that gene becomes less active, the light dims.

Huynh teamed up with Scoles, Stefan Pulst, M.D., Dr. Med, Chair of the Department of Neurology, and researchers at the National Center for Advancing Translational Sciences (NCATS) to put the light-producing cells to work, screening a vast collection of small molecules for their effects on the alpha-synuclein gene.

Millions of assays were performed to evaluate 155,885 different compounds using a high-throughput, robotic setup in an NCATS facility. A-443654 emerged from those analyses as a likely inhibitor of alpha-synuclein production. Postdoctoral researcher Mandi Gandelman, Ph.D., who joined the project after Dr. Huynh's death in 2018, conducted further tests and found that A-443654 not only dialed down activity of the alpha-synuclein gene in human cells-;including neurons derived from the cells of a patient with Parkinson's disease-;it also reduced the translation of RNA copies of the gene into alpha-synuclein protein.

Gandelman says there were other signs of the compound's therapeutic potential, as well. When alpha-synuclein aggregates accumulate and grow, they stress the systems that cells use to deal with broken, misshapen, or otherwise unwanted proteins. Gandelman's experiments indicated that A-443654 can alleviate this stress. That's a sign that cells are no longer overwhelmed by high levels of alpha-synuclein; it may also free up those systems to break down aggregates that have already formed. "We can stop production, but we also need to degrade what's already in aggregate," Gandelman says. "The more aggregated this is, the more toxic it becomes."

Further research is needed to determine whether A-443654 itself can be developed into a potential treatment for Parkinson's disease or other disorders involving alpha-synuclein aggregates. Meanwhile, the team also plans to explore additional molecules that their screen identified as potential inhibitors of alpha-synuclein production.

Source:
Journal reference:

Gandelman, M., et al. (2021) The AKT modulator A-443654 reduces α-synuclein expression and normalizes ER stress and autophagy. Journal of Biological Chemistry. doi.org/10.1016/j.jbc.2021.101191.

Comments

  1. ZaCloud StriFair ZaCloud StriFair United States says:

    So basically... instead of blood clots, it's nerve clots? :O
    Definitely a great potential breakthrough!

    Though I hope finding out what makes the alpha-synuclein stick together is being looked at too, of course. Like if there's a substance that causes them to stick together, and if fighting that substance might be a more direct course of action.

    (Like how blood-thinning may help against blood clots by reducing red blood cells, but it can cause anemia. But targeting the fibrin or platelets instead may be most ideal.)

    If the alpha-synuclein is just plain getting swollen and deformed, then yeah, reduction is important as a slightly further up-the-pipe treatment (And of course, eventually finding out what gosh darn silly factor is telling them to do that in the first place, and giving it a big ol' slap into submission).

    This'll just take a LOT of adjusting as it goes along, cuz I'd imagine reducing that stuff TOO much might interfere with nerve communication (if the currently-held belief that it helps with that remains true), and make the patient just kind of droop into nothing.

    Of course, that's what testing and research is for, to find the levels at which even a poison may become a valuable treatment or cure. Good luck!

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
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