Eclipse™ AF4 Asymmetric-Flow Field-Flow Fractionation System from Wyatt Technology

The Eclipse™AF4 solves many difficult separation, fractionation and sizing tasks for macromolecules, nanoparticles and colloids. Combined with a Wyatt DAWN® or miniDAWN™ MALS detector and Optilab® T-rEX™ refractive index detector, the Eclipse AF4 offers a uniquely versatile solution providing characterization of absolute molar mass and size as well as conformation and conjugation.

The primary advantage of asymmetric flow field-flow fractionation (AF4) over traditional SEC column chromatography is the ability to separate both soluble and colloidal components over a wide size range, from 1 nm to 1000 nm. AF4 utilizes an open separation channel with no stationary phase and very little surface area, and therefore generates almost no shear that can degrade sensitive samples. Since very little surface area is involved, adverse interactions between the sample and surfaces are minimized relative to sizing columns. Its resolution is similar to that of analytical ultracentifugation, but it surpasses even this benchmark-sizing tool when one considers its flexibility, speed, and ease of use. The Eclipse AF4 integrates seamlessly with various market-leading HPLC packages, such as Agilent, Thermo Scientific and Shimadzu, utilizing high-quality pumps, degassers, autosamplers and UV detectors while replacing just the column with an AF4 channel. The chassis can be ordered in three versions: for aqueous solvents (biocompatible), for organic solvents and in a metal-free version. For even more versatility consider the Eclipse DualTec, which supports automatic switching between AF4 and SEC as well as the hollow-fiber field-flow fractionation channel for nL-scale separations.