If you need to make quick decisions about potential food spoilage and contamination, the Maxwell® RSC PureFood Pathogen Kit offers a simple extraction protocol to obtain high-quality bacterial DNA from a variety of food sample types. The kit works with inhibiting sample types, and can lyse both Gram+ and Gram– bacteria, eliminating laborious sample preparation steps like enzymatic pretreatment.
The extracted DNA is ready for advanced downstream molecular analyses including NGS, serotyping, and identification of spoilage organisms. The high-performance Maxwell® chemistries coupled with the trusted benchtop Maxwell® RSC instrument allow you to purify bacterial DNA from food samples in as little as 40 minutes, giving you the ability to get answers more quickly.
High-Quality Bacterial DNA from Food Samples
- Automated isolation of DNA from raw or processed food samples in 40 minutes
- Works well with inhibiting sample types
- No need for labor-intensive sample processing
Isolate Pathogen DNA from Many Food Sample Types
This method has been used to isolate pathogen DNA from a number of different food samples including E. coli 0157:H7 from uncooked beef, Salmonella enterica from uncooked chicken, and Listeria monocytogenes from whole milk.
Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of E. coli 0157:H7 bacteria. The total DNA concentration was assessed using the QuantiFluor® ONE dsDNA System. Image credit: Promega
Comparison of total DNA and E. coli 0157:H7 DNA extracted from cilantro samples spiked with the indicated amounts of E. coli 0157:H7 bacteria. The concentration of E. coli 0157:H7 DNA was assessed by qPCR with specific primers. Image credit: Promega
Advantages
- Simple, automated protocol requires minimal hands-on steps.
- Avoid organic extractions and laborious sample preprocessing steps.
- Use an optimized system to extract bacterial DNA from many food samples