CellTiter-Glo® Luminescent Cell Viability Assay - To determine the number of viable cells in culture

The CellTiter-Glo® Luminescent Cell Viability Assay uses luminescence to accurately determine the number of viable cells in a culture.

  • Sequentially process plates exhibiting a highly consistent luminescence signal.
  • A uniform “add-mix-measure” methodology minimizes the number of stages involved.
  • Fewer cells are required per assay for higher levels of detection.

New! Use with the New MyGlo Reagent Reader from Promega. A customized 96-well MyGlo reagent reader system incorporating instrument, assay preparation, and data processing will help users speed up and simplify cell viability tests. MyGlo detects more than just assay signals. It walks users through every step of the CellTiter-Glo® experiments.

CellTiter-Glo® measures ATP, a key biomarker of cell health

The CellTiter-Glo® Luminescent Cell Viability Assay is a method for determining the number of viable cells in culture. It works by quantifying the ATP present, which is an indicator of metabolically active cells. This assay is compatible with multiwell formats, making it suitable for automated high-throughput screening (HTS), cell proliferation, and cytotoxicity assays.

The procedure involves adding a single reagent (CellTiter-Glo® Reagent) directly to cells cultured in a serum-supplemented medium, eliminating the need for cell washing, medium removal, or multiple pipetting steps. The system can detect as few as 15 cells per well in a 384-well format within 10 minutes after adding the reagent and mixing.

CellTiter-Glo® Luminescent Assay chemistry.

CellTiter-Glo® Luminescent Assay chemistry. Image Credit: Promega Corporation

CellTiter-Glo® Assay offers <10 cell sensitivity; Up to 5 logs linear range.

CellTiter-Glo® Assay offers <10 cell sensitivity; Up to 5 logs linear range. Image Credit: Promega Corporation

The uniform “add-mix-measure” method causes cell lysis and produces a luminescent signal whose intensity is directly related to the amount of ATP in the sample. The number of cells in the culture directly relates to the amount of ATP. Depending on the cell type and medium utilized, the CellTiter-Glo® Assay produces a "glow-type" luminescent signal with a half-life of usually more than five hours.

The longer half-life offers flexibility for batch or continuous processing of multiple plates and eliminates the requirement for reagent injectors. The unique homogeneous format avoids errors that could be introduced by other multi-step ATP measurement methods.

Utilized in numerous applications, the CellTiter-Glo® Luminescent Cell Viability Assay is a well-liked and reliable technique.

We perform knockdown experiments for noncoding RNAs on several different cell lines to identify novel cancer therapy targets. The CellTiter-Glo® Assay is an extremely easy-to-use reagent to address cell viability and obtain consistent and reproducible results.”

Anan Quan, Research Assistant, Beth Israel Deaconess Medical Center

The CellTiter-Glo® Assay provides a very robust, easy-to-use reagent for high-throughput screening of small molecules for antiviral activity against viruses such as SARS-CoV and SARS-CoV-2 that cause a cytopathic effect in cell lines.”

Dr. Colleen Jonsson, Director of the Regional Biocontainment Laboratory and Director of the Institute for the Study of Host-Pathogen Systems, University of Tennessee Health Science Center.


What is in the box?

Source: Promega Corporation

Item Part # Size
CellTiter-Glo® Substrate (lyophilized) G755A 1 × 1 vial
CellTiter-Glo® Buffer G756A 1 × 10ml

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