Life Science White Papers

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Life Science White Papers are in-depth articles aimed to educate and inform site visitors interested in life science research. Some of these papers are derived from sources where we have existing commercial relationships. Such articles are clearly labelled as sponsored content and are only accepted when the content is deemed to add value to the core editorial ethos of News Medical.

History and Product Range of Vitl Life Science Solutions

Vitl is a brand within the Integrated Technologies Ltd (ITL) group, which is a global product development and manufacturing company. The group has more than four decades of experience in instrument design and manufacturing projects, targeting medical devices, in vitro diagnostics (IVD), and laboratory equipment. The dedicated development team consists of ex-ITL engineers who have developed all Vitl products using this experience. This article discusses the team’s main milestones.

From Vitl Life Science Solutions 26 Apr 2016

Quantitative Measurement of Active Neutrophil Elastase Levels Using ProteaseTag™ Active NE Immunoassay

Lung function may be affected due to cycles of prolonged inflammation as well as chronic infection of the airways that lead to respiratory diseases.

From BMG LABTECH GmbH 18 Apr 2016

Flexible Technology Accelerates Diagnosis of Prostate Cancer

Prostate cancer is a common malignant disease seen in men, which accounts for almost one-third every year of all the new diagnoses made for cancer.

From Huron Digital Pathology 23 Mar 2016

Intracytoplasmic Sperm Injection Using Eppendorf Transferman® 4m Micromanipulator

Studies on assisted fertilization via micromanipulation techniques have gradually evolved from 1988 to the present day. The technique of intracytoplasmic sperm injection or ICSI (Figure 1) is a great benefit in providing treatment for infertile couples, especially those patients with male factor.

From Eppendorf 21 Mar 2016

Eppendorf TransferMan® 4r – All-Encompassing Manipulator for Genetic Engineering Techniques

The modification of laboratory animal genomes is carried out by micromanipulation of embryos or oocytes. The usual techniques of micromanipulation are: transcription activator-like effector nuclease (TALEN) into the cytoplasm into a pronucleus of early zygotes; or microinjection of nucleic acid constructs like larger bacterial artificial chromosomes (BAC), simple transgenes, and zinc finger (ZFN).

From Eppendorf 21 Mar 2016

Ramp Rate Data Alone Not Enough for Evaluation of PCR Thermal Cyclers

In order to compare various thermal cycler speeds, it is not enough to take into account only the heating ramp rate and cooling ramp rate available in the technical specifications, as these data do not give a picture of the actual run times.

From Eppendorf 21 Mar 2016

Dual Block Thermocycler Mastercycler® nexus GX2 Allows PCR Optimization, Offers High Flexibility

Flexible application options are essential for optimal utilization of laboratory instruments in many laboratories. In those laboratories where more than one user is dependent on a single instrument, flexible operation can result in usage frequency.

From Eppendorf 21 Mar 2016

Using Real-Time Infrared Chemical Imaging in Mass Spectrometry Tissue Analysis

Mass spectrometry (MS) is an analytical technique, which can be employed in studies involving the determination of the composition of complex biochemical samples.

From Daylight Solutions Inc 14 Mar 2016

PHERAstar® FSX Plate Reader Helps Improve Throughput of Screening Facilities

Screening facilities need to take necessary measures continuously for increased throughput, reduced sample expenditure and better control over cost.

From BMG LABTECH GmbH 22 Feb 2016

Using Secondary UV-VIS Filter Set to Evaluate the Functionality of Eppendorf BioPhotometer® and BioSpectrometer®

Whenever a sample is measured, the quality of the reading cannot exceed that of the measurement. Thus, it is vital to have a control on the sources of error that negatively impact the measurement or maintain them within defined limits. Errors can have technical causes or may be caused due to improper handling. Incorrect handling errors for photometric quantification includes sample concentration being too high or too low, wrongly programmed method factor, or insufficient sample mixing.

From Eppendorf 18 Feb 2016

Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration

This article shows how enzymatic activity can be effectively determined by means of the Eppendorf BioSpectrometer. The preliminary measurements were carried out by utilizing the “single λ-continuous” method for optimized measurement process. Based on these outcomes, the exact measurements of the enzyme activity were carried out. Linear regression was used to measure enzyme activities.

From Eppendorf 18 Feb 2016

Troubleshooting Guide for the Determination of Nucleic Acids Using Eppendorf BioPhotometer® D30 and Eppendorf BioSpectrometer®

This guide on troubleshooting will enable users to get reliable results when quantifying nucleic acids through various devices available in the Eppendorf photometer series (Figure 1). Therefore, the important factors which help to attain accurate measurements and problem-solving recommendations are given below. Figure 1 shows a pictorial example of Eppendorf BioPhotometer® D30.

From Eppendorf 18 Feb 2016

Standard Curve Generation for Colorimetric Assay in the Kinetic or Basic Eppendorf BioSpectrometer®

This article shows how the absorbance maxima of Ponceau-S is determined over a wavelength range of 220nm to 800nm using the Eppendorf BioSpectromenter®. The methods of analysis using this BioSpectrometer® are the detection of a maximum at 498nm while utilizing the function, Eppendorf “SpectraZoom”. Using the example of Ponceau-S, the colorimetric measurements were done by generating a standard curve. This standard programmed curve was then utilized to measure the concentration of unknown sample.

From Eppendorf 18 Feb 2016

Using Phenom ProX Desktop Scanning Electron Microscope in Microbiology Research

Biofilms are aggregates that are formed by bacteria, which grow on surfaces. Commonly found in environments, biofilms are highly resistant to antibiotics, and as such the mechanism and regulation of biofilms are being intensively studied by scientists across the globe.

From Thermo Fisher Scientific Phenom-World BV 8 Feb 2016

Detailed Considerations for Outfitting Preclinical In Vivo Imaging Laboratory

When setting up a preclinical in vivo imaging laboratory, a number of factors have to be taken into account. These will include the use of dedicated imaging systems and the predicted and accurate workflow of studies that will have to be performed.

From Bruker BioSpin - NMR, EPR and Imaging 1 Feb 2016

Characterization of Worm-Like Micelles Based on Rheology

Exploring the characteristics of worm-like micelles (WLMs) has been the focus of numerous academic and industry-based studies. The applications of WLMs are spread across a broad range of industries, from oil recovery to personal care.

From Malvern Panalytical 21 Jan 2016

Using DSC to Monitor Polyol-Induced Thermal Stability Increase in Antibodies

The steady growth of the biotechnology industry has increased the prominence of the study of protein aggregation. Monitoring and reducing the aggregation of proteins is an important aspect of protein therapeutics because aggregation is the potential cause of immunogenicity and reduced activity.

From Malvern Panalytical 21 Jan 2016

Advantages of Using DSC for Stability Screening of Engineered Proteins

Maintaining the required solubility, stability, efficacy and potency of an engineered biotherapeutic protein is critical after its expression in a recombinant system. Analysis and optimization of the characteristics of biotheraputic proteins such as monoclonal antibodies are performed during the engineering process.

From Malvern Panalytical 21 Jan 2016

Using Solid-State Standard Additions for Determining Active Ingredients in Solid Pharmaceutical Formulations

Determining an analyte in scenarios where the elimination or accurate reproduction of a matrix in a blank sample is not possible.

From Metrohm USA Inc. 18 Jan 2016

Using NIR Spectroscopy for Determining the Moisture, Fat, and Nitrogen in Human Feces

Health disorders like hepatic disorders or pancreatic insufficiency often result in malabsorption and maldigestion.

From Metrohm USA Inc. 18 Jan 2016

Life Science White Papers

History and Product Range of Vitl Life Science Solutions

Quantitative Measurement of Active Neutrophil Elastase Levels Using ProteaseTag™ Active NE Immunoassay

Flexible Technology Accelerates Diagnosis of Prostate Cancer

Intracytoplasmic Sperm Injection Using Eppendorf Transferman® 4m Micromanipulator

Eppendorf TransferMan® 4r – All-Encompassing Manipulator for Genetic Engineering Techniques

Ramp Rate Data Alone Not Enough for Evaluation of PCR Thermal Cyclers

Dual Block Thermocycler Mastercycler® nexus GX2 Allows PCR Optimization, Offers High Flexibility

Using Real-Time Infrared Chemical Imaging in Mass Spectrometry Tissue Analysis

PHERAstar® FSX Plate Reader Helps Improve Throughput of Screening Facilities

Using Secondary UV-VIS Filter Set to Evaluate the Functionality of Eppendorf BioPhotometer® and BioSpectrometer®

Using Eppendorf BioSpectrometer for the determination of enzyme activity and substrate concentration

Troubleshooting Guide for the Determination of Nucleic Acids Using Eppendorf BioPhotometer® D30 and Eppendorf BioSpectrometer®

Standard Curve Generation for Colorimetric Assay in the Kinetic or Basic Eppendorf BioSpectrometer®

Using Phenom ProX Desktop Scanning Electron Microscope in Microbiology Research

Detailed Considerations for Outfitting Preclinical In Vivo Imaging Laboratory

Characterization of Worm-Like Micelles Based on Rheology

Using DSC to Monitor Polyol-Induced Thermal Stability Increase in Antibodies

Advantages of Using DSC for Stability Screening of Engineered Proteins

Using Solid-State Standard Additions for Determining Active Ingredients in Solid Pharmaceutical Formulations

Using NIR Spectroscopy for Determining the Moisture, Fat, and Nitrogen in Human Feces

Evolving spectrophotometry for modern biopharma workflows

Scaling B-cell cloning workflows for antibody development

Fluidics in Modern Healthcare & Medical Device Innovation

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