Enzo expands its capability to perform simultaneous live-cell imaging of nuclear components

Enzo Life Sciences, a division of Enzo Biochem, Inc. (NYSE:ENZ), has added a novel new system to its CELLestial™ line of live cell analysis products, providing expanded capability to simultaneously image both the nucleus and the nucleolus in living cells, the Company announced today. Called the Total Nuclear-ID™ Green/Red Nucleolar/Nuclear Detection Kit, the new addition has been specifically optimized for demanding live cell microscopy applications.

The new system was featured in a recent issue of the publication “Microscopy Today,” in an article entitled “Red/Green Dual Fluorescence Detection of Both the Nucleus and Nucleolus in Living Cells.” In addition, Wayne Patton, Ph.D., Chief Scientific Officer for Enzo Life Sciences, presented a poster at the prestigious Microscopy and Microanalysis 2009 conference, held recently in Richmond, VA, on the utility of the kit for various drug discovery and basic cell biology applications.

"This complete system adds significant capabilities to our growing portfolio of live cell fluorescent probes to address unmet investigator needs,” said Dr. Patton. “The CELLestial™ product line encompasses a range of products optimized for various instrument platforms, including microscopes, microplate readers, flow cytometers and laser-scanning cytometers. Its introduction underscores our continuing commitment to introducing novel assay technologies that address rapidly expanding research fields and core applications in the Life Sciences."

The system contains proprietary dyes that are highly resistant to photobleaching and concentration quenching, ensuring strong, consistent fluorescence signal, even after extended viewing periods. It has been validated for use in live cell imaging applications, demonstrating appropriate response to treatment with well-characterized nucleolus-perturbing drugs. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition of transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying neoplastic cells.

As a world leader in non-radioactive probe technology, Enzo has now developed a wide range of products for the study of nucleic acids, as well as cellular components.

www.enzo.com