GoTaq 2-Step RT-qPCR System allows researchers to detect target genes

NewsGuard 100/100 Score

The new Promega GoTaq® 2-Step RT-qPCR System allows researchers to detect target genes expressed at low levels even with hard-to-amplify samples. Enhanced sensitivity of the two-step system extends the broad dynamic range of RNA quantitation and provides a robust, gene expression analysis tool.

GoTaq 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. The transcription system improves the yield of cDNA even in the presence of common inhibitors such as ethanol.

In the second step, cDNA is quantified using the GoTaq® qPCR Master Mix. Formulated with a hot-start DNA polymerase, the GoTaq qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.

The GoTaq 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript Reverse Transcription System with the ultra-bright fluorescence of GoTaq qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.

Source:

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Genetic key to milder COVID: Certain genes slash severity and death risk in older men