The new Promega GoTaq® 2-Step RT-qPCR System allows researchers to detect target genes expressed at low levels even with hard-to-amplify samples. Enhanced sensitivity of the two-step system extends the broad dynamic range of RNA quantitation and provides a robust, gene expression analysis tool.
GoTaq 2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA input. The transcription system improves the yield of cDNA even in the presence of common inhibitors such as ethanol.
In the second step, cDNA is quantified using the GoTaq® qPCR Master Mix. Formulated with a hot-start DNA polymerase, the GoTaq qPCR Master Mix is optimized for reproducibly specific, sensitive, highly efficient qPCR using the standard or fast mode of a real-time PCR instrument. The GoTaq qPCR Master Mix contains BRYT™ GREEN dye which can be twice as bright as of SYBR® Green I. The superior performance of these two steps allows researchers to observe earlier quantification cycle values over broader linear ranges.
The GoTaq 2-Step RT-qPCR System provides a ready-to-use kit for analyzing a wide range of RNA targets by combining the high-activity of GoScript Reverse Transcription System with the ultra-bright fluorescence of GoTaq qPCR Master Mix. The system is robust, requires little optimization, is flexible for a variety of applications, and carries the PCR performance guarantee.