Clontech Laboratories, Inc., a wholly-owned subsidiary of Takara Bio Inc., today announced the launch of the DNA SMART ChIP-Seq Kit. This kit adapts Clontech's patented SMART® technology in an innovative manner for use with low-input samples, including both dsDNA and ssDNA templates, to generate ChIP-seq libraries for NGS.
SMART (Switching Mechanism at 5' End of RNA Template) technology, when applied to RNA, allows for full-length cDNA synthesis with direct addition of a PCR adaptor. In the DNA SMART ChIP-Seq Kit, a novel, patent-pending adaptation of template switching technology is used to add Illumina®-specific adaptors directly to DNA. This produces ChIP-seq libraries without additional ligation or clean-up steps. The process has resulted in the simplification of library generation, cutting down protocol time significantly compared to traditional, ligation-based methods of adapter addition. The DNA SMART ChIP-Seq Kit generates libraries with good complexity and low redundancy from low-input samples (100 pg–10 ng) using a simple protocol that can be completed in half a day.
"Clontech is known as a leader in cDNA synthesis, and in recent years SMART technology has been widely acclaimed as the gold standard for sensitive transcriptome analysis from low-input samples, including single cells," said Carol Lou, General Manager of Clontech Laboratories, Inc. "With the DNA SMART ChIP-Seq Kit we are able to expand the benefits of SMART technology into other NGS applications. Our focus has been providing new solutions to customers, enabling them to work with challenging samples. This kit allows us to address the needs of those researchers working with low inputs of either dsDNA or ssDNA for NGS applications."
The launch of the DNA SMART ChIP-Seq Kit continues the Clontech® tradition of supporting good science by providing new tools to meet the ever-changing needs of life science researchers.
SOURCE Clontech Laboratories, Inc.