Chromatrap describes methodologies to increase ChIP efficiency

Chromatrap, a business unit of Porvair Sciences, has published a new technical article that discusses and describes methodologies to increase Chromatin Immunoprecipitation (ChIP) efficiency by making sure chromatin in your samples is sheared to the correct size range.

The authors describe how for every chromatin preparation it is essential to check the chromatin is sheared to fragments between 100-500 bp. They emphasise using a microfluidics platform is the most accurate measure of quantifying DNA.

Problems associated with over or under sheared Chromatin fragments are discussed and a protocol for optimised enzymatic shearing described. As some cells are resistant to lysis the authors also describe an optimised sonication methodology to ensure a successful ChIP result.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Chromatrap® - Porvair Sciences Ltd. (2019, June 20). Chromatrap describes methodologies to increase ChIP efficiency. News-Medical. Retrieved on December 01, 2022 from https://www.news-medical.net/news/20160719/Chromatrap-describes-methodologies-to-increase-ChIP-efficiency.aspx.

  • MLA

    Chromatrap® - Porvair Sciences Ltd. "Chromatrap describes methodologies to increase ChIP efficiency". News-Medical. 01 December 2022. <https://www.news-medical.net/news/20160719/Chromatrap-describes-methodologies-to-increase-ChIP-efficiency.aspx>.

  • Chicago

    Chromatrap® - Porvair Sciences Ltd. "Chromatrap describes methodologies to increase ChIP efficiency". News-Medical. https://www.news-medical.net/news/20160719/Chromatrap-describes-methodologies-to-increase-ChIP-efficiency.aspx. (accessed December 01, 2022).

  • Harvard

    Chromatrap® - Porvair Sciences Ltd. 2019. Chromatrap describes methodologies to increase ChIP efficiency. News-Medical, viewed 01 December 2022, https://www.news-medical.net/news/20160719/Chromatrap-describes-methodologies-to-increase-ChIP-efficiency.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of News Medical.
Post a new comment
Post
You might also like...
Improving Workflow in Life Science Labs